Simultaneous quantification of six flavonoids of Rhus verniciflua Stokes using matrix solid-phase dispersion via high-performance liquid chromatography coupled with photodiode array detector.

A simple and efficient matrix solid-phase dispersion via high-performance liquid chromatography coupled with a photodiode array detector was developed to analyze the following flavonoids of Rhus verniciflua Stokes: fisetin, fustin, butein, sulfuretin, garbanzol, and quercetin. The optimum conditions for the procedure was the use of Zeolite Socony Mobil-twenty-two molecular sieves as the adsorbent, sample:adsorbent ratio of 2:5, grinding for 3 min, and use of 8 mL of 70% methanol:water as the elution solvent. The method was validated for linearity, precision, reproducibility, limit of detection, and limit of quantification. The method exhibited excellent linearity for all six flavonoids. The intra- and interday precisions over a range of concentrations were below 3.0% and limits of quantification for the six flavonoids were 0.16 and 0.50 μg/mL. Compared with other published methods, the proposed method was more effective, rapid, and required less reagents. Therefore, the combination of matrix solid-phase dispersion and high-performance liquid chromatography coupled with photodiode array detector showed excellent reproducibility and simplicity and could be suitable for the extraction and quantification of multiple flavonoids in R. verniciflua Stokes samples.