Institute of Chemical Industry of Forest Products, CAF, Nanjing, P.R. China.
National Engineering Laboratory for Biomass Chemical Utilization, Key and Open Laboratory of Forest Chemical Engineering, SFA, Nanjing, P.R. China.
J Sep Sci. 2020 Dec;43(23):4281-4288. doi: 10.1002/jssc.202000749. Epub 2020 Oct 15.
A simple and efficient matrix solid-phase dispersion via high-performance liquid chromatography coupled with a photodiode array detector was developed to analyze the following flavonoids of Rhus verniciflua Stokes: fisetin, fustin, butein, sulfuretin, garbanzol, and quercetin. The optimum conditions for the procedure was the use of Zeolite Socony Mobil-twenty-two molecular sieves as the adsorbent, sample:adsorbent ratio of 2:5, grinding for 3 min, and use of 8 mL of 70% methanol:water as the elution solvent. The method was validated for linearity, precision, reproducibility, limit of detection, and limit of quantification. The method exhibited excellent linearity for all six flavonoids. The intra- and interday precisions over a range of concentrations were below 3.0% and limits of quantification for the six flavonoids were 0.16 and 0.50 μg/mL. Compared with other published methods, the proposed method was more effective, rapid, and required less reagents. Therefore, the combination of matrix solid-phase dispersion and high-performance liquid chromatography coupled with photodiode array detector showed excellent reproducibility and simplicity and could be suitable for the extraction and quantification of multiple flavonoids in R. verniciflua Stokes samples.
建立了一种简单高效的基质固相分散-高效液相色谱-光电二极管阵列检测法，用于分析盐肤木中的以下黄酮类化合物：非瑟酮、漆黄素、杨梅素、二氢漆黄素、garbanzol 和槲皮素。该方法的最佳条件为：采用沸石 Socony Mobil-twenty-two 分子筛作为吸附剂，样品与吸附剂的比例为 2:5，研磨 3 min，用 8 mL 70%甲醇：水作为洗脱溶剂。该方法对六种黄酮类化合物进行了线性、精密度、重现性、检测限和定量限的验证。该方法对所有六种黄酮类化合物均表现出良好的线性。在一定浓度范围内，日内和日间精密度均低于 3.0%，六种黄酮类化合物的定量限分别为 0.16 和 0.50 μg/mL。与其他已发表的方法相比，该方法更有效、快速，所需试剂更少。因此，基质固相分散-高效液相色谱-光电二极管阵列检测法显示出良好的重现性和简单性，可用于盐肤木样品中多种黄酮类化合物的提取和定量。
