Institute of Pharmaceutical Science and Technology and College of Pharmacy, Hanyang University, Ansan, Gyeonggi-do 426-791, Republic of Korea.
Food Chem. 2013 Dec 15;141(4):3813-9. doi: 10.1016/j.foodchem.2013.06.068. Epub 2013 Jun 25.
A simple, sensitive, and precise reversed-phase liquid chromatographic method was developed for the quantitative determination of 4 bioactive phenolic compounds (gallic acid, fustin, fisetin, and sulfuretin) from the stem extract of Rhus verniciflua stokes. Chromatographic analysis was performed on a Capcell Pak C18 column (150 × 4.6 mm, 3 μm) with a mobile phase consisting of 0.1% formic acid and 90% acetonitrile at a flow rate of 1 mL/min. Quantitation was performed using a UV-vis detector at 260 nm. The method was validated in terms of selectivity, linearity, accuracy, precision, and recovery. Excellent linear behavior was observed over the investigated concentration range (10-500 μg/mL for gallic acid, fustin, and fisetin; 0.5-100 μg/mL for sulfuretin) with correlation coefficient (r(2)) values >0.99. The intra- and inter-day precision over the concentration range of compounds was less than 6.65% (relative standard deviation) and the accuracy was between 92.42% and 103.62%. The mean recoveries for all the analytes were more than 92.18%. This method was successfully applied for the analysis of bioactive phenolic compounds in the R. verniciflua extracts.
建立了一种简单、灵敏、精确的反相液相色谱法，用于定量测定从漆树茎提取物中提取的 4 种生物活性酚类化合物（没食子酸、漆黄素、非瑟酮和黄颜木素）。色谱分析在 Capcell Pak C18 柱（150×4.6mm，3μm）上进行，流动相由 0.1%甲酸和 90%乙腈组成，流速为 1mL/min。采用 UV-vis 检测器在 260nm 处进行定量。该方法在选择性、线性、准确性、精密度和回收率方面进行了验证。在所研究的浓度范围内（没食子酸、漆黄素和非瑟酮为 10-500μg/mL；黄颜木素为 0.5-100μg/mL），观察到良好的线性行为，相关系数（r（2））值大于 0.99。在化合物浓度范围内，日内和日间精密度小于 6.65%（相对标准偏差），准确度在 92.42%至 103.62%之间。所有分析物的平均回收率均大于 92.18%。该方法成功应用于漆树提取物中生物活性酚类化合物的分析。
