Kapitulnik J, Gelboin H V, Guengerich F P, Jacobowitz D M
Neuroscience. 1987 Mar;20(3):829-33. doi: 10.1016/0306-4522(87)90243-0.
The presence of cytochrome P-450 in rat brain was studied by immunohistochemistry, using antibodies to cytochrome P-450 purified from livers of phenobarbital- or 3-methylcholanthrene-treated rats. Immunoreactive nerves were observed only in brain sections incubated with immunoglobulin-G to 3-methylcholanthrene-induced cytochrome P-450. This immunoreactivity was abolished by preabsorption of the antibody with highly purified rat liver cytochrome P-450c, the major cytochrome P-450 isozyme induced by 3-methylcholanthrene, but was not affected by other cytochrome P-450 isozymes induced by phenobarbital, isosafrole or pregnenolone-16 alpha-carbonitrile. The most abundant concentration of nerve fibers with cytochrome P-450 immunoreactivity was observed in the globus pallidus. Immunoreactive fibers were also observed in the caudate putamen, amygdala, septum, ventromedial nucleus of the hypothalamus, medial forebrain bundle, ansa lenticularis, and ventromedial portion of the internal capsule and crus cerebri. Cell bodies with cytochrome P-450 immunoreactivity were observed in the caudate putamen and in the perifornical area of the hypothalamus. The cytochrome P-450 immunoreactive fibers in the globus pallidus and caudate putamen do not appear to emanate from cell bodies in the substantia nigra, since there was no reduction in the density of these fibers after unilateral stereotaxic electrolytic destruction of the substantia nigra (zona compacta and reticulata). Our data suggest that these striatal nerve processes are derived from cell bodies within the caudate putamen itself. The present results indicate that rat brain contains a form of cytochrome P-450 with antigenic relatedness to the hepatic 3-methylcholanthrene-inducible cytochrome P-450c.(ABSTRACT TRUNCATED AT 250 WORDS)
采用从苯巴比妥或3 - 甲基胆蒽处理的大鼠肝脏中纯化的细胞色素P - 450抗体，通过免疫组织化学方法研究大鼠脑中细胞色素P - 450的存在情况。仅在与针对3 - 甲基胆蒽诱导的细胞色素P - 450的免疫球蛋白 - G孵育的脑切片中观察到免疫反应性神经。用高度纯化的大鼠肝脏细胞色素P - 450c（3 - 甲基胆蒽诱导的主要细胞色素P - 450同工酶）预先吸收抗体后，这种免疫反应性消失，但不受苯巴比妥、异黄樟素或孕烯醇酮 - 16α - 腈诱导的其他细胞色素P - 450同工酶的影响。在苍白球中观察到具有细胞色素P - 450免疫反应性的神经纤维浓度最高。在尾状壳核、杏仁核、隔区、下丘脑腹内侧核、内侧前脑束、豆状袢、内囊腹侧部分和大脑脚中也观察到免疫反应性纤维。在尾状壳核和下丘脑穹窿周区观察到具有细胞色素P - 450免疫反应性的细胞体。苍白球和尾状壳核中的细胞色素P - 450免疫反应性纤维似乎并非源自黑质中的细胞体，因为在单侧立体定向电解破坏黑质（致密部和网状部）后，这些纤维的密度没有降低。我们的数据表明，这些纹状体神经突起源自尾状壳核自身内的细胞体。目前的结果表明，大鼠脑中含有一种与肝脏3 - 甲基胆蒽诱导的细胞色素P - 450c具有抗原相关性的细胞色素P - 450形式。（摘要截短于250字）
