Jessell T M, Emson P C, Paxinos G, Cuello A C
Brain Res. 1978 Sep 8;152(3):487-98. doi: 10.1016/0006-8993(78)91104-6.
The topographical projections of substance P pathways from the caudateputamen and globus pallidus to the pars compacta and pars reticulata of the substantia nigra have been investigated in the rat using immunohistochemical and radioimmunoassay techniques and compared with the projections of GABA nergic striatal neurones. Unilateral vertical knife cuts through the anterior and posterior striatum have shown the majority of substance P-containing neurones which project to the substantia nigra to originate in the most rostral part of the caudate-putamen. This projection appears to innervate the pars reticulata and pars compacta of the substantia nigra to a similar extent. A separate projection of substance P-containing neurones to the substantia nigra appears to originate in the globus pallidus. Undercutting the cerebral cortex which overlies the corpus striatum did not affect the substance P content of the globus pallidus or substantia nigra. However, there appears to be an additional substance P projection from the basal ganglia to the entopeduncular nucleus. In contrast, GABA-containing neurones which project to the substantia nigra are mainly located in more caudal parts of the caudate-putamen and in the globus pallidus. There is a marked differentiation in the region of the substantia nigra innervated by GABA cells originating in the rostral and caudal parts of the corpus striatum. Rostrally situated neurones project almost exclusively to the pars reticulata, while neurones in the caudal part of the caudate-putamen and globus pallidus project to both the pars compacta and pars reticulata. These results suggest that there is a partial topographical separation of the sites of origin of substance P- and GABA-containing neurones which project to the substantia nigra.
运用免疫组织化学和放射免疫分析技术，在大鼠身上研究了从尾状核壳核和苍白球到黑质致密部和网状部的P物质通路的局部投射，并与γ-氨基丁酸（GABA）能纹状体神经元的投射进行了比较。通过在前后纹状体进行单侧垂直刀切割，发现投射到黑质的大多数含P物质的神经元起源于尾状核壳核最前端部分。这种投射似乎对黑质网状部和致密部的支配程度相似。另一组含P物质的神经元向黑质的投射似乎起源于苍白球。切除覆盖纹状体的大脑皮层并不影响苍白球或黑质的P物质含量。然而，似乎存在一条从基底神经节到内囊核的额外P物质投射。相比之下，投射到黑质的含GABA的神经元主要位于尾状核壳核更靠后的部分以及苍白球。起源于纹状体前后部的GABA细胞所支配的黑质区域存在明显差异。位于前部的神经元几乎只投射到网状部，而尾状核壳核和苍白球后部的神经元则投射到致密部和网状部。这些结果表明，投射到黑质的含P物质和含GABA的神经元的起源部位存在部分局部性分离。
