鼻窦腔骨窗提升作为皮质骨移植物对愈合的影响：兔模型的组织形态计量学研究。

The Influence on Healing of Bony Window Elevated Inward in the Sinus Cavity as Cortical Bone Graft: A Histomorphometric Study in Rabbit Model.

出版信息

Int J Oral Maxillofac Implants. 2020 Sep/Oct;35(5):879-887. doi: 10.11607/jomi.8226.

DOI:10.11607/jomi.8226

PMID:32991637

Abstract

PURPOSE

To evaluate the influence on healing of the bony window elevated inward in the sinus cavity as a cortical bone graft.

MATERIALS AND METHODS

Eighteen rabbits were included in the experiment. At the test sites (bony window), the antrostomy was prepared and the remaining bony window was elevated together with the sinus mucosa. At the control sites, the bony window was gently detached before the sinus mucosa elevation and discarded. The space obtained was grafted with deproteinized bovine bone mineral (DBBM). A collagen membrane was positioned on the antrostomy at both sides. The rabbits were euthanized after 2, 4, and 8 weeks in groups of six each. Histologic analyses in different regions of the elevated space were carried out, and a Wilcoxon test was used to estimate differences. Microcomputed tomography (microCT) analyses were also performed.

RESULTS

After 2 weeks of healing, higher proportions of new bone were found in the test group compared with the control group due to the higher amount of bone formed in the region subjacent to the sinus mucosa. In this region, higher amounts of new bone were also found in the test group after 4 (P = .028) and 8 weeks of healing (P = .345). After 8 weeks of healing, the percentage of new bone was higher at the control sites compared with the test sites, with the proportions being 25.4% ± 3.2% and 21.3% ± 6.1%, respectively. In this period of evaluation, the bony window contributed with 20.1% ± 5.3% of vital bone in the test group. A fraction of 60.4% ± 10.8% of its surface was surrounded by new bone. In the microCT analysis, after 8 weeks of healing, fractions of 22.3% ± 1.6% and 22.2% ± 0.7% of bone were found in the test and control groups, respectively.

CONCLUSION

The presence of the bony window positively influenced the healing in the elevated space, especially in the submucosa region. The bony window was vital and incorporated into newly formed bone.

摘要

目的

评估鼻窦腔内向内提升的骨窗作为皮质骨移植物对愈合的影响。

材料和方法

将 18 只兔子纳入实验。在试验部位（骨窗），制备窦口并将剩余的骨窗与鼻窦黏膜一起抬起。在对照部位，在抬起鼻窦黏膜之前轻轻分离骨窗并丢弃。用脱蛋白牛骨矿物质（DBBM）填充获得的空间。在两侧窦口放置胶原膜。每组 6 只兔子分别在 2、4 和 8 周后处死。对不同抬高空间区域进行组织学分析，并使用 Wilcoxon 检验估计差异。还进行了微计算机断层扫描（microCT）分析。

结果

在愈合后 2 周，由于在黏膜下区域形成的骨量较高，实验组的新骨比例高于对照组。在该区域，在愈合后 4 周（P =.028）和 8 周（P =.345）时，实验组的新骨量也更高。在愈合后 8 周，对照组的新骨比例高于实验组，分别为 25.4%±3.2%和 21.3%±6.1%。在评估期间，实验组的骨窗在测试组中提供了 20.1%±5.3%的活骨。其表面的 60.4%±10.8%被新骨包围。在 microCT 分析中，在愈合后 8 周时，实验组和对照组的骨分数分别为 22.3%±1.6%和 22.2%±0.7%。