Yamada Rihito, Xavier Samuel Porfirio, Nakajima Yasushi, Silva Erick Ricardo, Botticelli Daniele, Teranishi Yuki, Baba Shunsuke
Department of Oral Implantology, School of Dentistry, Osaka Dental University, 8-1 Kuzuhahanazonocho, Hirakata 573-1121, Osaka, Japan.
Department of Oral and Maxillofacial Surgery and Periodontology, Faculty of Dentistry of Ribeirão Preto, University of São Paulo, Av. do Café-Subsetor Oeste-11 (N-11), Ribeirão Preto 14040-904, SP, Brazil.
Dent J (Basel). 2025 Jan 2;13(1):19. doi: 10.3390/dj13010019.
This investigation focused on the influence of collagen on the integrity of the Schneiderian membrane during maxillary sinus augmentation in a rabbit model. The aim of this study was to elucidate the relationship between membrane integrity and bone regeneration in augmented maxillary sinuses using collagenated and non-collagenated grafts, through detailed histological and histomorphometric analyses. In this forward-looking, randomized, split-mouth design, bilateral maxillary sinus augmentation was conducted on 12 rabbits. One sinus was filled with deproteinized bovine bone material (DBBM) as the grafting material (non-collagenated; control), while a combination of DBBM particles integrated with 10% porcine-derived type I collagen was used in the contralateral sinus (collagenated; test). Histological analyses were carried out following healing periods of 2 and 12 weeks. At the 2-week time point, six sites of thinned mucosa (<40 µm) and no perforations were observed in the collagenated group, while twenty-one thinned mucosa sites ( = 0.027 between test and control) and two perforations ( = 0.175 between test and control) were found in the non-collagenated group. After 12 weeks of healing, the number of sites with thinned mucosa was 20 in the collagenated group and 19 in the non-collagenated group, with four perforations observed in each group. These perforations were distributed across three sinuses in the collagenated group and two sinuses in the non-collagenated group. No statistically significant differences were found between the groups. : The addition of 10% collagen to deproteinized bovine bone mineral initially provided protection against mucosal thinning and perforation after 2 weeks of healing. However, by week 12, this protective effect diminished, resulting in similar rates of mucosal thinning and perforations in both groups.
本研究聚焦于在兔上颌窦提升模型中，胶原蛋白对上颌窦黏膜完整性的影响。本研究的目的是通过详细的组织学和组织形态计量学分析，阐明使用含胶原蛋白和不含胶原蛋白的移植物时，上颌窦提升后黏膜完整性与骨再生之间的关系。在这项前瞻性、随机、双侧对照设计中，对12只兔子进行双侧上颌窦提升。一侧上颌窦填充脱蛋白牛骨材料（DBBM）作为移植材料（不含胶原蛋白；对照组），而对侧上颌窦使用与10%猪源I型胶原蛋白混合的DBBM颗粒（含胶原蛋白；试验组）。在愈合2周和12周后进行组织学分析。在2周时间点，含胶原蛋白组观察到6个黏膜变薄（<40 µm）且无穿孔的部位，而不含胶原蛋白组发现21个黏膜变薄部位（试验组与对照组之间P = 0.027）和2处穿孔（试验组与对照组之间P = 0.175）。愈合12周后，含胶原蛋白组黏膜变薄部位数量为20个，不含胶原蛋白组为19个，每组均观察到4处穿孔。这些穿孔在含胶原蛋白组分布于3个鼻窦，在不含胶原蛋白组分布于2个鼻窦。两组之间未发现统计学显著差异。结论：在脱蛋白牛骨矿物质中添加10%胶原蛋白，在愈合2周后最初可防止黏膜变薄和穿孔。然而，到第12周时，这种保护作用减弱，导致两组黏膜变薄和穿孔发生率相似。
