Department of Microbiology, University of Wisconsin-La Crosse, La Crosse, Wisconsin 54601, USA.
(ORCID: https://orcid.org/0000-0003-1568-1999 [X.L.]).
J Food Prot. 2020 Oct 1;83(10):1782-1788. doi: 10.4315/0362-028X.JFP-19-410.
This study evaluated the efficacy of copper alloy surfaces for inactivation of Tulane virus (TV), assessed by plaque assay and porcine gastric mucin-conjugated magnetic bead (PGM-MB) binding assay, followed by quantitative reverse transcription PCR (PGM-MB-RT-qPCR assay). In addition, the efficacy of a copper surface for inactivation of human norovirus (HuNoV) GII.4 Sydney and GI.3B Potsdam strains was evaluated by PGM-MB-RT-qPCR assay. Results of time-dependent inactivation of viruses on copper, bronze, and brass coupons revealed that 15 min of surface treatments of each of the copper and copper alloys achieved >4-log reduction of purified TV, as assessed by plaque assay, while up to 20 min of copper alloy surface treatments only achieved ∼2-log reduction, as assessed by PGM-MB-RT-qPCR assay. As assessed by PGM-MB-RT-qPCR assay, 10 min of copper surface treatments achieved reductions of 3 and 4 log units for HuNoVs GII.4 Sydney and GI.3B Potsdam, respectively. Results from this study suggest that even though PGM-MB-RT-qPCR assay underestimated the efficacy of copper alloy surface inactivation of TV, copper alloy surfaces were able to effectively inactivate TV and HuNoVs. Therefore, copper alloys can be used as a preventive measure to prevent HuNoV infection and are an effective surface treatment for HuNoVs.
本研究通过噬斑试验和猪胃黏膜黏蛋白结合磁珠（PGM-MB）结合试验评估了铜合金表面对图兰病毒（TV）的灭活效果，并用 PGM-MB-RT-qPCR 试验评估了铜合金表面对人类诺如病毒（HuNoV）GII.4 悉尼株和 GI.3B 波茨坦株的灭活效果。病毒在铜、青铜和黄铜片上的时间依赖性灭活结果表明，铜和铜合金表面处理 15 分钟即可实现纯化 TV 的 >4 对数减少，噬斑试验评估结果如此，而 PGM-MB-RT-qPCR 试验评估结果显示，铜合金表面处理长达 20 分钟仅实现了约 2 对数减少。PGM-MB-RT-qPCR 试验评估结果显示，铜表面处理 10 分钟即可分别减少 HuNoVs GII.4 悉尼株和 GI.3B 波茨坦株 3 和 4 个对数单位。本研究结果表明，尽管 PGM-MB-RT-qPCR 试验低估了铜合金表面对 TV 的灭活效果，但铜合金表面能够有效灭活 TV 和 HuNoVs。因此，铜合金可用作预防 HuNoV 感染的措施，也是 HuNoVs 的有效表面处理方法。
