Department of Food Science, College of Agriculture, Tunghai University, Taichung City, Taiwan.
Department of Traditional Chinese Medicine, Chang Gung Memorial Hospital, Keelung City, Taiwan.
J Agric Food Chem. 2020 Oct 21;68(42):11790-11801. doi: 10.1021/acs.jafc.0c02513. Epub 2020 Oct 7.
Siamenoside I is a rare mogroside in Swingle and has become one of the target ingredients in natural sweetener production. However, the complex structure of siamenoside I has hindered its production in various ways. Here, a yeast cell that produces a specific β-glucosidase for siamenoside I conversion from mogroside V was constructed, and the enzymes were coelectrospun with poly(vinyl alcohol) followed by phenylboronic acid cross-linking to provide potential usage in the batch production process of Siamenoside I. A central composite design (CCD)-response surface methodology (RSM) was used to find the optimum coelectrospinning parameters. The pH stability and sodium dodecyl sulfate tolerance increased for the entrapped enzymes, and positive correlations between the fiber diameter and enzymatic activity were confirmed. The batch process showed an average siamenoside I production rate of 118 ± 0.08 mg L h per gram of fiber. This is the first research article showing specific siamenoside I production on enzyme-loaded electrospun fibers.
西马苷 I 是一种稀有的甜菊糖苷，已成为天然甜味剂生产的目标成分之一。然而，西马苷 I 的复杂结构以各种方式阻碍了其生产。在这里，构建了一种酵母细胞，该细胞可产生用于西马苷 V 转化的特定β-葡萄糖苷酶，并将酶与聚乙烯醇共静电纺丝，然后用苯硼酸交联，为西马苷 I 的批生产过程提供了潜在用途。采用中心组合设计（CCD）-响应面法（RSM）寻找最佳共静电纺丝参数。固定化酶的 pH 稳定性和十二烷基硫酸钠耐受性提高，并且证实了纤维直径与酶活性之间的正相关关系。批处理过程中，每克纤维的西马苷 I 平均产量为 118 ± 0.08mg L h。这是第一篇关于在负载酶的静电纺纤维上进行特定西马苷 I 生产的研究文章。
