Department of Life and Environmental Sciences, Università Politecnica delle Marche, Via Brecce Bianche, 60131 Ancona, Italy.
Clinica Ostetrica Ginecologica, Università Politecnica delle Marche, Ospedale G. Salesi, Via F. Corridoni 11, 60121 Ancona, Italy.
Int J Mol Sci. 2020 Sep 27;21(19):7124. doi: 10.3390/ijms21197124.
Different Follicle Stimulating Hormone (FSH) formulation and Luteinizing Hormone (LH) are used in Assisted Reproductive Technology (ART) to induce follicles development and oocytes maturation, but it is still under debate which protocol is to be preferred. In the present study, the different effects on cumulus cells (CCs) of three controlled ovarian stimulation (COS) protocols, based on urinary FSH, recombinant FSH, or human Menopausal Gonadotropin (hMG) administration, were assessed. CCs were obtained from 42 normal-responders women undergoing COS, randomly divided into three groups according to the used gonadotropin formulation. Differences were found in the expression of genes belonging to the endocannabinoid system (the receptors , and , and the enzymes involved in the metabolisms of anandamide, and , and 2-acylglycerol, and ); consistently, changes in lipid (α, and ) and carbohydrate ( and ) metabolisms, in CCs' macromolecules composition (highlighted by Fourier Transform Infrared Microspectroscopy, FTIRM), and in the number of retrieved oocytes were found. For the first time, statistically significant evidence on the differences related to each COS protocol on the endocannabinoid system, metabolism and macromolecular composition of CCs was found, representing a proof of concept to be further confirmed in a larger cohort of patients.
不同的卵泡刺激素（FSH）制剂和黄体生成素（LH）用于辅助生殖技术（ART）中诱导卵泡发育和卵母细胞成熟，但哪种方案更优仍存在争议。本研究评估了基于尿促性腺激素、重组 FSH 或人绝经期促性腺激素（hMG）给药的三种控制性卵巢刺激（COS）方案对卵丘细胞（CC）的不同影响。从 42 名正常反应者中获得 CCs，他们正在接受 COS，根据使用的促性腺激素制剂将其随机分为三组。在属于内源性大麻素系统的基因表达中发现了差异（受体 、 和 ，以及参与花生四烯酸、 和 、2-酰基甘油、 和 代谢的酶）；一致地，在 CCs 的大分子组成（傅里叶变换红外显微镜，FTIRM）中的脂质（α 和 ）和碳水化合物（ 和 ）代谢、变化以及可回收卵母细胞数量方面也发现了变化。这是首次在与每个 COS 方案相关的内源性大麻素系统、CCs 的代谢和大分子组成方面发现具有统计学意义的差异证据，为在更大的患者队列中进一步证实这一概念提供了依据。
