Parente Paola, Covelli Claudia, Zanelli Magda, Trombetta Domenico, Carosi Illuminato, Carbonelli Cristiano, Sperandeo Marco, Mastracci Luca, Biancofiore Giovanni, Zizzo Maurizio, Taurchini Marco, Ascani Stefano, Graziano Paolo
Pathology Unit, Fondazione IRCCS Casa Sollievo della Sofferenza, 71013 San Giovanni Rotondo, Italy.
Pathology Unit, Azienda Unità Sanitaria Locale-IRCCS di Reggio Emilia, 42123 Reggio Emilia, Italy.
Diagnostics (Basel). 2020 Sep 25;10(10):748. doi: 10.3390/diagnostics10100748.
The diagnosis of lymphoma requires surgical specimens to perform morphological evaluation, immunohistochemical and molecular analyses. Ultrasound-guided fine needle aspiration may represent an appropriate first approach to obtain cytological samples in impalpable lesions and/or in patients unsuitable for surgical procedures. Although cytology has intrinsic limitations, the cell block method may increase the possibility of achieving an accurate diagnosis.
We retrospectively selected a total of 47 ultrasound-guided fine needle aspiration and drainage samples taken from patients with effusion and deep-seated lesions which are clinically suspicious in terms of malignancy.
In 27 cases, both cell block and conventional cytology were performed: 21/27 cell blocks were adequate for the diagnosis of lymphoma and suitable for immunocytochemistry and molecular analyses vs. 12/20 samples to which only conventional cytology was applied. Moreover, in five patients we were able to make a diagnosis of Hodgkin lymphoma with the cell block (CB) technique.
Contrary to conventional cytology, the cell block method may allow immunocytochemistry and molecular studies providing useful information for the diagnosis and subtypization of lymphoma in patients unsuitable for surgical procedure or with deep-seated lesions or extra-nodal diseases; additionally, it is a daily, simple and helpful approach. Moreover, we describe the usefulness of cell blocks in the diagnosis of Hodgkin lymphoma.
淋巴瘤的诊断需要手术标本进行形态学评估、免疫组化和分子分析。超声引导下细针穿刺抽吸可能是获取不可触及病变和/或不适合手术的患者细胞学样本的合适首选方法。尽管细胞学有其固有的局限性，但细胞块法可能会增加做出准确诊断的可能性。
我们回顾性选择了总共47例超声引导下细针穿刺抽吸和引流样本，这些样本取自临床上怀疑为恶性的有积液和深部病变的患者。
在27例病例中，同时进行了细胞块和传统细胞学检查：27例中有21例细胞块足以诊断淋巴瘤并适用于免疫细胞化学和分子分析，而仅应用传统细胞学检查的20个样本中有12例。此外，在5例患者中，我们能够通过细胞块（CB）技术诊断霍奇金淋巴瘤。
与传统细胞学不同，细胞块法可进行免疫细胞化学和分子研究，为不适合手术、有深部病变或结外疾病的患者的淋巴瘤诊断和亚型分类提供有用信息；此外，它是一种日常、简单且有用的方法。此外，我们描述了细胞块在霍奇金淋巴瘤诊断中的作用。
