Nuñez-Gómez Vanesa, Baenas Nieves, Navarro-González Inma, García-Alonso Javier, Moreno Diego A, González-Barrio Rocío, Periago-Castón Mª Jesús
Department of Food Technology, Food Science and Nutrition, Faculty of Veterinary Sciences, Regional Campus of International Excellence "Campus Mare Nostrum", Biomedical Research Institute of Murcia (IMIB-Arrixaca-UMU), University Clinical Hospital "Virgen de la Arrixaca", University of Murcia, Espinardo, 30100 Murcia, Spain.
Phytochemistry and Healthy Foods Lab, Department of Food Science and Technology, CEBAS-CSIC, University Campus of Espinardo-25, E-30100 Murcia, Spain.
Foods. 2020 Sep 26;9(10):1371. doi: 10.3390/foods9101371.
Broccoli is a source of bioactive compounds that provide an important nutritional value. The content of these compounds can vary depending on agronomic and environmental conditions, as well as on elicitation. In this study, three crop trials were carried out to evaluate the effects of the cultivation season, the application of different dosages of methyl-jasmonate (MeJA) on the overall quality and on the total content of bioactive compounds of 'Parthenon' broccoli cultivated under the field conditions of southeastern Spain. Color parameters, chlorophyll content, total phenolic compounds, total flavonoids and antioxidant activity were measured to evaluate the overall quality. Moreover, individual carotenoids, phenolic compounds and glucosinolates were evaluated by high performance liquid chromatography with diode array detection (HPLC-DAD) and high performance liquid chromatography equipped with diode array detector coupled to mass spectrometer using electro spray ionization (HPLC-DAD-ESI/MS). The content of total carotenoids, phenolic compounds and glucosinolates were higher in autumn compared with spring, showing increases of 2.8-fold, 2-fold and 1.2-fold, respectively. Moreover, a double application of MeJA increased the contents of total carotenoids, phenolic compounds and glucosinolates by 22%, 32% and 39%, respectively, relative to the untreated samples. Considering our results, the controlled and timely application of 250 µM MeJA to the aerial parts of the plants four days before harvest, on two consecutive days, seems to be a valid agronomic strategy to improve the health-promoting capacity of Parthenon broccoli, without compromising its overall quality.
西兰花是生物活性化合物的来源，具有重要的营养价值。这些化合物的含量会因农艺和环境条件以及诱导作用而有所不同。在本研究中，进行了三项作物试验，以评估栽培季节、不同剂量茉莉酸甲酯（MeJA）的施用对在西班牙东南部田间条件下种植的‘帕特农神庙’西兰花的整体品质和生物活性化合物总含量的影响。通过测量颜色参数、叶绿素含量、总酚化合物、总黄酮和抗氧化活性来评估整体品质。此外，通过配备二极管阵列检测的高效液相色谱法（HPLC-DAD）以及配备二极管阵列检测器并与使用电喷雾电离的质谱仪联用的高效液相色谱法（HPLC-DAD-ESI/MS）对单个类胡萝卜素、酚类化合物和硫代葡萄糖苷进行了评估。秋季总类胡萝卜素、酚类化合物和硫代葡萄糖苷的含量高于春季，分别增加了2.8倍、2倍和1.2倍。此外，与未处理的样品相比，双重施用MeJA使总类胡萝卜素、酚类化合物和硫代葡萄糖苷的含量分别增加了22%、32%和39%。考虑到我们的研究结果，在收获前四天连续两天向植株地上部分施用250µM的MeJA，似乎是一种有效的农艺策略，可提高‘帕特农神庙’西兰花的健康促进能力，同时不影响其整体品质。
