Yang Tingyu, Fu Jilagamazhi, Ma Liang, Du Hong, Yue Xiaoqing, Zhao Bosheng, Wang Cuiyan
College of Chemical Engineering, Inner Mongolia University of Technology, Hohhot 010051, China; Inner Mongolia Engineering Research Center for CO(2) Capture and Utilization, Hohhot 010051, China.
College of Chemical Engineering, Inner Mongolia University of Technology, Hohhot 010051, China; Inner Mongolia Engineering Research Center for CO(2) Capture and Utilization, Hohhot 010051, China.
Mater Sci Eng C Mater Biol Appl. 2020 Sep;114:111019. doi: 10.1016/j.msec.2020.111019. Epub 2020 May 5.
In biomineralization, organisms have the abilities to produce biominerals with superior properties. One of the most attractive features of biominerals is the presence of the proteins consisting of different contents of amino acids in crystals. In the present work, L-phenylalanine (Phe) was used as an additive for the controllable crystallization of calcium carbonate (CaCO). The obtained CaCO crystals were characterized by field emission scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), elemental analysis and high-resolution transmission electron microscopy (HRTEM). The experimental results suggest that single calcite crystals are formed at low Phe concentrations. High concentrations of Phe inhibit the nucleation and growth of calcite, and promote the formation of vaterite crystals with solid or hollow structures. The morphology and crystal form of CaCO are also significantly affected by the flow rate of CO. After that, a possible mechanism (competition mechanism) action of Phe in the formation of CaCO is proposed. Finally, the effects of temperature on the formation of vaterite were determined to explore the growth mechanism of hexagonal vaterite. The work of controlling the preparation of CaCO crystals in the presence of Phe will help us to imitate and learn nature, and bring new insights into understanding bionics. Meanwhile, it provides a new method for the synthesis of CaCO biomaterials with different crystal forms and morphologies.
在生物矿化过程中，生物体具有生成具有优异性能生物矿物的能力。生物矿物最吸引人的特征之一是晶体中存在由不同氨基酸含量组成的蛋白质。在本工作中，L-苯丙氨酸（Phe）被用作碳酸钙（CaCO₃）可控结晶的添加剂。通过场发射扫描电子显微镜（SEM）、X射线衍射（XRD）、傅里叶变换红外光谱（FTIR）、元素分析和高分辨率透射电子显微镜（HRTEM）对所得碳酸钙晶体进行了表征。实验结果表明，在低Phe浓度下形成了方解石单晶。高浓度的Phe抑制方解石的成核和生长，并促进具有实心或空心结构的球霰石晶体的形成。碳酸钙的形态和晶型也受到CO₂流速的显著影响。在此之后，提出了Phe在碳酸钙形成过程中的一种可能作用机制（竞争机制）。最后，确定了温度对球霰石形成的影响，以探索六方球霰石的生长机制。在Phe存在下控制碳酸钙晶体制备的工作将有助于我们模仿和学习自然，并为理解仿生学带来新的见解。同时，它为合成具有不同晶型和形态的碳酸钙生物材料提供了一种新方法。
