Gomułka Krzysztof, Liebhart Jerzy, Lange Andrzej, Mêdrala Wojciech
Department of Internal Medicine, Pneumology and Allergology, Wroclaw Medical University, Wroclaw, Poland.
Laboratory of Clinical Immunology, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.
Postepy Dermatol Alergol. 2020 Aug;37(4):584-589. doi: 10.5114/ada.2020.95954. Epub 2020 Jun 25.
Vascular endothelial growth factor (VEGF) is an angiogenic cytokine and a potential stimulator of permeability and lung neovascularization in asthmatics. It also plays an important role in the development of airway remodelling and in activation of many cells, including basophils.
To reveal the possible role of VEGF in the activation of basophils in asthmatics - subgroups with and without irreversible bronchoconstriction. Protein CD203c on the basophils surface was used as the activation marker. To define the possible pathway of basophils VEGF-activation, the influence of a genetic factor (polymorphism del18/ins -2549 -2567 in the VEGF-promoter region) was also considered.
Our study involved 82 patients with asthma (40 patients without and 42 patients with irreversible bronchoconstriction) and a group of 40 controls. The flow cytometric methods with anti-CD203c in the samples of basophils with increasing concentrations of VEGF was used for analysis of their activity. Genotyping for VEGF-promoter region was performed by PCR-based methods.
Patients with asthma and del/del genotype showed more significant differences in the basophils activation after stimulation with increasing concentrations of VEGF than asthmatics with ins/ins and ins/del genotype ( = 0.023) and controls with del/del genotype ( = 0.0006).
Raised basophils VEGF-activation is characteristic for examined patients with asthma and might be associated with presence of polymorphism del18/ins -2549 -2567 in the VEGF-promoter region. Furthermore, it may contribute to the development of airways remodelling - this pathway has not been considered yet.
血管内皮生长因子（VEGF）是一种血管生成细胞因子，是哮喘患者通透性和肺新血管形成的潜在刺激因子。它在气道重塑的发展以及包括嗜碱性粒细胞在内的许多细胞的激活中也起着重要作用。
揭示VEGF在哮喘患者（有和无不可逆支气管收缩的亚组）嗜碱性粒细胞激活中的可能作用。嗜碱性粒细胞表面的蛋白CD203c用作激活标志物。为了确定嗜碱性粒细胞VEGF激活的可能途径，还考虑了遗传因素（VEGF启动子区域的多态性del18/ins -2549 -2567）的影响。
我们的研究涉及82例哮喘患者（40例无不可逆支气管收缩患者和42例有不可逆支气管收缩患者）以及40例对照组。使用抗CD203c的流式细胞术方法，对VEGF浓度增加的嗜碱性粒细胞样本进行活性分析。通过基于PCR的方法对VEGF启动子区域进行基因分型。
与具有ins/ins和ins/del基因型的哮喘患者（P = 0.023）以及具有del/del基因型的对照组（P = 0.0006）相比，具有del/del基因型的哮喘患者在VEGF浓度增加刺激后嗜碱性粒细胞激活方面表现出更显著差异。
嗜碱性粒细胞VEGF激活升高是所检查哮喘患者的特征，可能与VEGF启动子区域的多态性del18/ins -2549 -2567有关。此外，它可能有助于气道重塑的发展——这一途径尚未被考虑。
