Jiang Ping, Yang Xin, Li Yuanli, Chen Juan
Department of Orthopaedics Affiliated Hospital of North Sichuan Medical College Nanchong China.
Food Sci Nutr. 2020 Aug 9;8(9):4708-4716. doi: 10.1002/fsn3.1587. eCollection 2020 Sep.
The aim of this study is to explain the effects and mechanism of miRNA-216 in osteosarcoma. We firstly evaluated the PTEN expression in 30 pairs of tumor and adjacent tissues which were from the 30 osteosarcoma patients. In the following cell experiments, we measured the cell proliferation, cell cycle, cell invasion, and migration abilities of NC (normal control) group, BL (blank) group, siRNA (miRNA-216 inhibitor) group, and siRNA+PTEN inhibitor group. Furthermore, we measured the relative protein expression of difference groups by WB to explain the mechanism of miRNA-216 in osteosarcoma. The PTEN was confirmed the target gene of miRNA-216 by double luciferase target test. In conclusion, miRNA-216 was an oncogene in osteosarcoma. miRNA-216 knockdown had effects to suppress cancer cell proliferation, invasion and migration and improve cell apoptosis by keeping in G1 phase via PTEN.
本研究旨在阐明miRNA-216在骨肉瘤中的作用及机制。我们首先检测了30例骨肉瘤患者的30对肿瘤组织及癌旁组织中PTEN的表达情况。在接下来的细胞实验中，我们检测了正常对照组（NC）、空白组（BL）、siRNA组（miRNA-216抑制剂组）和siRNA+PTEN抑制剂组的细胞增殖、细胞周期、细胞侵袭及迁移能力。此外，我们通过蛋白质免疫印迹法（WB）检测不同组别的相关蛋白表达，以阐明miRNA-216在骨肉瘤中的作用机制。通过双荧光素酶靶标实验证实PTEN是miRNA-216的靶基因。综上所述，miRNA-216在骨肉瘤中是一种癌基因。敲低miRNA-216可通过PTEN使细胞停滞于G1期，从而抑制癌细胞的增殖、侵袭和迁移，并促进细胞凋亡。
