miRNA-216 knockdown has effects to suppress osteosarcoma via stimulating PTEN.

The aim of this study is to explain the effects and mechanism of miRNA-216 in osteosarcoma. We firstly evaluated the PTEN expression in 30 pairs of tumor and adjacent tissues which were from the 30 osteosarcoma patients. In the following cell experiments, we measured the cell proliferation, cell cycle, cell invasion, and migration abilities of NC (normal control) group, BL (blank) group, siRNA (miRNA-216 inhibitor) group, and siRNA+PTEN inhibitor group. Furthermore, we measured the relative protein expression of difference groups by WB to explain the mechanism of miRNA-216 in osteosarcoma. The PTEN was confirmed the target gene of miRNA-216 by double luciferase target test. In conclusion, miRNA-216 was an oncogene in osteosarcoma. miRNA-216 knockdown had effects to suppress cancer cell proliferation, invasion and migration and improve cell apoptosis by keeping in G1 phase via PTEN.