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植物性蛋白质零食:发酵和超声处理对最终产品特性的影响。

Plant-based proteinaceous snacks: Effect of fermentation and ultrasonication on end-product characteristics.

作者信息

Gunasekaran Yasaswini Kooniambedu, Lele Vita, Sakiene Vytaute, Zavistanaviciute Paulina, Zokaityte Egle, Klupsaite Dovile, Bartkevics Vadims, Guiné Raquel P F, Bartkiene Elena

机构信息

Lithuanian University of Health Sciences Kaunas Lithuania.

Centre of Food Chemistry University of Latvia Riga Latvia.

出版信息

Food Sci Nutr. 2020 Aug 3;8(9):4746-4756. doi: 10.1002/fsn3.1705. eCollection 2020 Sep.

Abstract

The study aimed at the development of a sufficient technology to improve sensory, textural, physical, and microbiological properties of peas snacks (Ps) using solid-state fermentation (SSF) and submerged fermentation (SMF) with two different lactic acid bacteria (LAB) strains ( LUHS210 and LUHS245) for 24 hr and ultrasonication (10, 20, and 30 min). To ensure safety of the used technologies, microbiological characteristics and biogenic amines (BAs) content in treated Ps were analyzed. Additionally, a different salt content (3.6 and 1.0 g/100 g) was used for snacks preparation. The obtained results revealed that used treatments reduced enterobacteria in Ps, while in fermented Ps, yeast/moulds were not found. Ps with the lower salt content were more acidic and harder (0.90 mJ), and there was a significant effect ( < .05) due to the fermentation method, LAB strains, and ultrasonication on the texture of final product. Different salt content significantly affected the color coordinates of the Ps tested ( < .05). The predominant biogenic amines in Ps were phenylethylamine and spermidine. However, the reduction of some BAs after samples fermentation was observed. To conclude, acceptable formulations of Ps can be obtained with 1.0 g/100 g salt, and by using fermentation, as the end-product is more attractive to consumers than those prepared with 3.6 g/100 g salt and using ultrasonication.

摘要

本研究旨在开发一种充分的技术,利用固态发酵(SSF)和深层发酵(SMF),使用两种不同的乳酸菌(LAB)菌株(LUHS210和LUHS245)处理24小时,并进行超声处理(10、20和30分钟),以改善豌豆零食(Ps)的感官、质地、物理和微生物特性。为确保所用技术的安全性,分析了处理后Ps的微生物特性和生物胺(BAs)含量。此外,在制备零食时使用了不同的盐含量(3.6和1.0克/100克)。所得结果表明,所用处理方法减少了Ps中的肠杆菌,而在发酵后的Ps中未发现酵母/霉菌。盐含量较低的Ps更酸且更硬(0.90毫焦),发酵方法、LAB菌株和超声处理对最终产品的质地有显著影响(<0.05)。不同的盐含量对测试的Ps的颜色坐标有显著影响(<0.05)。Ps中主要的生物胺是苯乙胺和亚精胺。然而,观察到样品发酵后一些BAs有所减少。总之,使用1.0克/100克盐并通过发酵可以获得可接受的Ps配方,因为最终产品比使用3.6克/100克盐并进行超声处理制备的产品对消费者更有吸引力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba56/7500785/0ec2e380e8a8/FSN3-8-4746-g001.jpg

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