Ma Yanhong, Li Yahui, Zhang Hongzhi, Wang Ying, Wu Caie, Huang Wuyang
Institute of Agro-Product Processing Jiangsu Academy of Agricultural Sciences Nanjing China.
School of Food and Biological Engineering Jiangsu University Zhenjiang China.
Food Sci Nutr. 2020 Aug 10;8(9):5095-5106. doi: 10.1002/fsn3.1810. eCollection 2020 Sep.
Blueberries have great beneficial effects due to high level of anthocyanins, especially malvidin. Hepatic stellate cells (HSCs) can be activated and increase excessive extracellular matrix (ECM) components, which play a central role in liver fibrogenesis. Therefore, activated HSC's apoptosis can be induced to recover liver fibrosis. Malvidin's effects on apoptosis in rat activated hepatic stellate T6 cells (HSC-T6) in vitro were investigated here. High concentration of malvidin was found to significantly induce apoptosis, activate caspase-3, increase malondialdehyde, upregulate Bax, but downregulate Bcl-2. Moreover, malvidin upregulated the protein levels of some endoplasmic reticulum stress (ERS)-typical markers, including caspase-12, glucose-regulated protein 78 (GRP78), and CCAAT/enhancer-binding protein (C/EBP) homologous protein (CHOP), suggesting that malvidin induced HSC apoptosis by the ERS apoptosis pathway as well as the mitochondrial-dependent pathway. These findings indicated that blueberry anthocyanins, especially malvidin, could induce activated hepatic stellate cell apoptosis and might act as one kind of functional food ingredient or a novel nutraceutical beneficial for liver health.
蓝莓因含有高水平的花青素，尤其是锦葵色素，而具有诸多有益功效。肝星状细胞（HSCs）可被激活并增加过量的细胞外基质（ECM）成分，这些成分在肝纤维化形成过程中起核心作用。因此，诱导活化的肝星状细胞凋亡可恢复肝纤维化。本研究在此探讨了锦葵色素对体外培养的大鼠活化肝星状T6细胞（HSC-T6）凋亡的影响。研究发现，高浓度的锦葵色素可显著诱导细胞凋亡、激活半胱天冬酶-3、增加丙二醛含量、上调Bax蛋白表达，但下调Bcl-2蛋白表达。此外，锦葵色素上调了一些内质网应激（ERS）典型标志物的蛋白水平，包括半胱天冬酶-12、葡萄糖调节蛋白78（GRP78）和CCAAT/增强子结合蛋白（C/EBP）同源蛋白（CHOP），这表明锦葵色素通过ERS凋亡途径以及线粒体依赖途径诱导肝星状细胞凋亡。这些研究结果表明，蓝莓花青素，尤其是锦葵色素，可诱导活化的肝星状细胞凋亡，可能作为一种功能性食品成分或新型营养保健品，对肝脏健康有益。
