小分子调控的不稳定结构域设计使用方案
Protocol for Designing Small-Molecule-Regulated Destabilizing Domains for Use.
机构信息
Department of Ophthalmology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA.
Department of Pharmacology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA.
出版信息
STAR Protoc. 2020 Sep 18;1(2). doi: 10.1016/j.xpro.2020.100069. Epub 2020 Jul 24.
The use of destabilizing domains (DDs) to conditionally control the abundance of a protein of interest (POI) through a small-molecule stabilizer has gained increasing traction both and . Yet there are specific considerations for the development and accurate control of user-defined POIs via DDs, as well as the identification of novel (and potentially synergistic) small-molecule stabilizers. Here, we describe a platform for achieving these goals. For complete details on the use and execution of this protocol, please refer to Ramadurgum et al. (2020).
通过小分子稳定剂来使用非稳定结构域（DDs）有条件地控制目标蛋白（POI）的丰度，这在 和 中都得到了越来越多的关注。然而，通过 DDs 开发和准确控制用户定义的 POI 以及识别新的（可能协同作用的）小分子稳定剂需要考虑特定的因素。在这里，我们描述了一个实现这些目标的平台。有关此方案的详细使用和执行信息，请参见 Ramadurgum 等人（2020 年）。
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