大肠杆菌中的重组蛋白表达：历史视角
Recombinant Protein Expression in E. coli : A Historical Perspective.
作者信息
Gileadi Opher
机构信息
Structural Genomics Consortium, University of Oxford, Old Road Campus Research Building, Roosevelt Drive, Headington, Oxford, OX3 7DQ, UK.
出版信息
Methods Mol Biol. 2017;1586:3-10. doi: 10.1007/978-1-4939-6887-9_1.
PMID:28470595
Abstract
This introductory chapter provides a brief historical survey of the key elements incorporated into commonly used E. coli-based expression systems. The highest impact in expression technology is associated with innovations that were based on extensively studied biological systems, and where the tools were widely distributed in the academic community.
摘要
本章引言部分简要回顾了常用大肠杆菌表达系统中关键要素的历史。表达技术方面的最大影响与基于深入研究的生物系统的创新相关，这些创新所涉及的工具在学术界广泛传播。
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