Natarajan Harini, Crowley Andrew R, Butler Savannah E, Xu Shiwei, Weiner Joshua A, Bloch Evan M, Littlefield Kirsten, Wieland-Alter Wendy, Connor Ruth I, Wright Peter F, Benner Sarah E, Bonny Tania S, Laeyendecker Oliver, Sullivan David, Shoham Shmuel, Quinn Thomas C, Larman H Benjamin, Casadevall Arturo, Pekosz Andrew, Redd Andrew D, Tobian Aaron A R, Ackerman Margaret E
Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Dartmouth College, Hanover, NH, USA.
Thayer School of Engineering, Dartmouth College, Hanover, NH, USA.
medRxiv. 2020 Sep 18:2020.09.16.20196154. doi: 10.1101/2020.09.16.20196154.
Convalescent plasma has emerged as a promising COVID-19 treatment. However, the humoral factors that contribute to efficacy are poorly understood. This study functionally and phenotypically profiled plasma from eligible convalescent donors. In addition to viral neutralization, convalescent plasma contained antibodies capable of mediating such Fc-dependent functions as complement activation, phagocytosis and antibody-dependent cellular cytotoxicity against SARS-CoV-2. These activities expand the antiviral functions associated with convalescent plasma and together with neutralization efficacy, could be accurately and robustly from antibody phenotypes. These results suggest that high-throughput profiling could be used to screen donors and plasma may provide benefits beyond neutralization.
康复期血浆已成为一种有前景的新冠病毒治疗方法。然而，对其疗效起作用的体液因素却知之甚少。本研究对符合条件的康复期献血者的血浆进行了功能和表型分析。除病毒中和作用外，康复期血浆还含有能够介导补体激活、吞噬作用和针对新冠病毒的抗体依赖性细胞毒性等Fc依赖性功能的抗体。这些活性扩展了与康复期血浆相关的抗病毒功能，并且与中和效力一起，可以从抗体表型中准确而有力地推断出来。这些结果表明，高通量分析可用于筛选献血者，且血浆可能提供超出中和作用的益处。
