Department of Agri-Food Industry, Food and Nutrition, 'Luiz de Queiroz' College of Agriculture, University of São Paulo, Avenida Pádua Dias, 13418-900, Piracicaba, SP, Brazil.
Department of Physiological Sciences, Piracicaba Dental School, University of Campinas, Avenida Limeira 901, Areião, 13414-018, Piracicaba, SP, Brazil and Graduate Program in Biological Sciences, Federal University of Alfenas, MG, Brazil.
Food Funct. 2020 Oct 21;11(10):8905-8917. doi: 10.1039/d0fo01763a.
Brazilian native fruits (BNF) have aroused interest of researchers and consumers for their great human health benefits. In this study, five BNF (Byrsonima lancifolia, Campomanesia phaea, Jacaratia spinosa, Solanum alternatopinnatum and Acnistus arborescens) were tested for their polyphenolic compounds by LC-ESI-MS/MS, reactive species deactivation (ROO˙, O2˙-, HOCl and NO˙), anti-inflammatory properties in vivo, and in vitro antimicrobial activity - with determination of putative mechanism(s) of action. Eighty-one polyphenols were identified, which exhibited a significant capacity to deactivate both ROS and RNS. C. phaea extract had the highest capacity to scavenge ROO˙ (68.94 μmol TE per g), O2˙- (IC50: 575.36 μg mL-1) and NO˙ (IC50: 16.96 μg mL-1), which may be attributed to the presence of ellagitanins. B. lancifolia decreased neutrophil influx into the peritoneal cavity of mice by 50% as compared to carrageenan and reduced Candida albicans biofilm viability by 3 log10 possibly due to complexation with cell membrane ergosterol. In summary, the BNF presented herein are good sources of bioactive compounds with positive effects on deactivation of biological reactive species, as well as with anti-inflammatory and antimicrobial activities, which can be altogether highly beneficial to human health.
巴西本土水果（BNF）因其对人类健康的巨大益处而引起了研究人员和消费者的兴趣。在这项研究中，五种 BNF（Byrsonima lancifolia、Campomanesia phaea、Jacaratia spinosa、Solanum alternatopinnatum 和 Acnistus arborescens）通过 LC-ESI-MS/MS 进行了多酚化合物测试、活性物质失活（ROO˙、O2˙-、HOCl 和 NO˙）、体内抗炎特性和体外抗菌活性-并确定了可能的作用机制。鉴定出 81 种多酚，它们具有显著的失活 ROS 和 RNS 的能力。C. phaea 提取物具有最高的 ROO˙清除能力（68.94 μmol TE/g）、O2˙-（IC50：575.36 μg/mL）和 NO˙（IC50：16.96 μg/mL），这可能归因于鞣花单宁的存在。B. lancifolia 将小鼠腹腔内中性粒细胞的流入减少了 50%，与角叉菜胶相比，并降低了白色念珠菌生物膜的活力 3 个对数，可能是由于与细胞膜麦角固醇的络合。总之，本文中提到的 BNF 是生物活性化合物的良好来源，具有生物活性物质失活、抗炎和抗菌活性的积极作用，对人类健康非常有益。
