Chuncheon Center, Korea Basic Science Institute (KBSI), Chuncheon, 24341, Republic of, Korea.
National Institute of Food and Drug Safety Evaluation Herbal Medicinal Products Division, Ministry of Food and Drug Safety, Chungcheongbuk-do, 28159, Republic of, Korea.
Chem Biodivers. 2020 Nov;17(11):e2000470. doi: 10.1002/cbdv.202000470. Epub 2020 Nov 3.
Two new oligostilbenes, caragasinins D and E, along with four known compounds, kobophenol A, α-viniferin, wistin, and 5-hydroxy-2-[(4-hydroxyphenyl)acetyl]-3-methoxybenzoic acid, were isolated from the roots of Caragana sinica. These compounds were spectroscopically analyzed for their structures and configurations and compared with existing data. The configurations of caragasinins D and E were elucidated by H-NMR spectroscopy, CD spectroscopy, and time-dependent density-functional theory simulated ECD spectral data. All six compounds were evaluated for their inhibitory activity against neuraminidase (NA) from Clostridium perfringens. Among the tested compounds, 5-hydroxy-2-[(4-hydroxyphenyl)acetyl]-3-methoxybenzoic acid demonstrated statistically significant NA inhibitory activity, which was comparable to the positive control, mangiferin.
从柠条根部分离得到两个新的二苯乙烯类化合物（caragasinins D 和 E）和四个已知化合物（kobophenol A、α-viniferin、wistin 和 5-hydroxy-2-[(4-hydroxyphenyl)acetyl]-3-methoxybenzoic acid）。通过光谱分析确定了这些化合物的结构和构型，并与现有数据进行了比较。通过 1H-NMR 光谱、圆二色谱和基于时间密度泛函理论的模拟电子圆二色谱数据确定了 caragasinins D 和 E 的构型。评估了所有六种化合物对产气荚膜梭菌神经氨酸酶（NA）的抑制活性。在测试的化合物中，5-hydroxy-2-[(4-hydroxyphenyl)acetyl]-3-methoxybenzoic acid 表现出统计学上显著的 NA 抑制活性，与阳性对照芒果苷相当。
