Qazvin University of Medical Sciences, Qazvin, 34197-59811, Iran.
Hematology, Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran 14155-6559, Iran.
Exp Oncol. 2020 Sep;42(3):188-191. doi: 10.32471/exp-oncology.2312-8852.vol-42-no-3.15180.
Osteopontin (OPN) plays a critical role in cell proliferation and drug resistance in cancer treatment and hematological malignancies. In T cell acute lymphoblastic leukemia, most initial therapies can induce remission while some patients then relapse and do not respond well to chemotherapy. The sesquiterpene lactone parthenolide (PTL) can induce apoptosis in a variety of cancer cell lines via inhibition of pro-inflammatory transcription factor nuclear factor kappa B and has anti-tumor activity in acute lymphoblastic leukemia treatment.
To study the role of OPN in conferring in vitro resistance to PTL in Jurkat cells.
Jurkat cells were cultured with 8-20 μm PTL for 48 h. Transfection with OPN siRNA was provided. Apoptosis assays were performed with Annexin V-Alexa Fluor-488/PI. Quantitative real-time polymerase chain reaction was used to measure OPN gene expression using the 2-2 method.
PTL has cytotoxic and apoptotic effect on Jurkat cells with IC values of 16.1 μm, and growth inhibition effect of PTL does not differ significantly in combination with OPN-siRNA. OPN gene expression is not affected by PTL.
Parthenolide induces apoptosis in Jurkat cells, but inhibition of osteopontin gene expression with siRNA does not reduce apoptotic effect of parthenolide.
骨桥蛋白（OPN）在癌症治疗和血液恶性肿瘤中的细胞增殖和耐药性中发挥着关键作用。在 T 细胞急性淋巴细胞白血病中，大多数初始疗法可以诱导缓解，但有些患者随后复发，对化疗反应不佳。倍半萜内酯（PTL）可通过抑制促炎转录因子核因子κB 诱导多种癌细胞系凋亡，并在急性淋巴细胞白血病治疗中具有抗肿瘤活性。
研究 OPN 在赋予 Jurkat 细胞对 PTL 体外耐药性中的作用。
用 8-20 μm PTL 培养 Jurkat 细胞 48 小时。提供 OPN siRNA 转染。用 Annexin V-Alexa Fluor-488/PI 进行凋亡测定。用 2-2 法定量实时聚合酶链反应测量 OPN 基因表达。
PTL 对 Jurkat 细胞具有细胞毒性和凋亡作用，IC 值为 16.1 μm，与 OPN-siRNA 联合使用时，PTL 的生长抑制作用无显著差异。PTL 不影响 OPN 基因表达。
PTL 诱导 Jurkat 细胞凋亡，但用 siRNA 抑制骨桥蛋白基因表达不会降低 PTL 的凋亡作用。
