Abe Takaaki, Okamoto Shunya, Taniguchi Akinobu, Fukui Michiyasu, Yamaguchi Akinobu, Utsumi Yuichi, Ukita Yoshiaki
Integrated Graduate School of Medicine, Engineering, and Agricultural Sciences, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi, Japan.
Anal Methods. 2020 Oct 22;12(40):4858-4866. doi: 10.1039/d0ay01459a.
In this paper, we report on the demonstration of a portable immunoassay system consisting of a small centrifugal microfluidic device driver (bento box) and a centrifugal microfluidic device made of polypropylene and fabricated by injection molding. The bento box consists of a cheap DC motor and an Arduino microcontroller. It has a simple structure and is the size of a bento box, that is, 150 × 150 × 100 (W × D × H) mm3. The developed device can automatically execute an enzyme-linked immunosorbent assay (ELISA) process under a steady rotating condition because it was designed based on the principle of CLOCK, which we previously presented. Here, we first executed an ELISA using a system consisting of the bento box and a device made of polydimethylsiloxane (PDMS) and compared it with a servo-controlled device driver. It was confirmed that the results of the bento box were consistent with those of the servo-controlled device driver. The limit of detection (LOD) using the bento box was 0.759 ng ml-1. Therefore, the controllability of the bento box was demonstrated. Next, we evaluated the injection-molded device through multi-step fluid control. We confirmed, through real-time observation of the device, that accurate flow control in the designed ELISA procedure was executed. Lastly, ELISA was employed for the measurements of mouse IgG using the system consisting of the bento box and the polypropylene device. The system performed all fluidic controls within 12 min; we confirmed the specificity of the system, and the LOD was 0.320 ng ml-1.
在本文中，我们报告了一种便携式免疫分析系统的演示，该系统由一个小型离心微流控设备驱动器（便当盒）和一个由聚丙烯制成并通过注塑成型制造的离心微流控设备组成。便当盒由一个廉价的直流电机和一个Arduino微控制器组成。它结构简单，尺寸为便当盒大小，即150×150×100（宽×深×高）mm³。所开发的设备能够在稳定旋转条件下自动执行酶联免疫吸附测定（ELISA）过程，因为它是基于我们之前提出的CLOCK原理设计的。在这里，我们首先使用由便当盒和聚二甲基硅氧烷（PDMS）制成的设备组成的系统进行了ELISA，并将其与伺服控制的设备驱动器进行了比较。结果证实，便当盒的结果与伺服控制的设备驱动器的结果一致。使用便当盒的检测限（LOD）为0.759 ng ml⁻¹。因此，证明了便当盒的可控性。接下来，我们通过多步流体控制对注塑成型设备进行了评估。通过对设备的实时观察，我们确认在设计的ELISA程序中执行了精确的流量控制。最后，使用由便当盒和聚丙烯设备组成的系统对小鼠IgG进行ELISA测量。该系统在12分钟内完成了所有流体控制；我们确认了该系统的特异性，检测限为0.320 ng ml⁻¹。
