Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Department of Laboratory Medicine, 6500 HB Nijmegen, the Netherlands.
Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Department of Laboratory Medicine, 6500 HB Nijmegen, the Netherlands.
Cell Rep. 2020 Sep 29;32(13):108204. doi: 10.1016/j.celrep.2020.108204.
Well-timed interaction of correctly functioning maternal immune cells is essential to facilitate healthy placenta formation, because the uterine immune environment has to tolerate the semi-allogeneic fetus and allow adequate trophoblast invasion. Here, we assess the uterine immune signature before and during pregnancy. Extensive supervised and unsupervised flow cytometry clustering strategies not only show a general increase in immune memory throughout pregnancy but also reveal the continuous presence of B cells. Contrary to the belief that B cells are merely a consequence of uterine pathology, decidual B cells produce IL-10 and are found to be localized in clusters, together with Foxp3 T cells. Our findings therefore suggest a role for B cells in healthy pregnancy.
母体免疫细胞的适时相互作用对于促进健康的胎盘形成至关重要，因为子宫内的免疫环境必须耐受半同种异体胎儿并允许足够的滋养细胞浸润。在这里，我们评估了妊娠前和妊娠期间的子宫免疫特征。广泛的监督和无监督的流式细胞术聚类策略不仅显示了整个怀孕期间免疫记忆的普遍增加，而且还揭示了 B 细胞的持续存在。与 B 细胞仅仅是子宫病理学后果的观点相反，蜕膜 B 细胞产生 IL-10，并被发现与 Foxp3 T 细胞一起定位于簇中。因此，我们的研究结果表明 B 细胞在健康妊娠中发挥作用。
