Centre for DNA Fingerprinting and Diagnostics, Uppal, Hyderabad 500039, India.
Centre for DNA Fingerprinting and Diagnostics, Uppal, Hyderabad 500039, India.
Cell Rep. 2020 Sep 29;32(13):108202. doi: 10.1016/j.celrep.2020.108202.
Bacteriophytochromes are the most abundant and ubiquitous light-sensing receptors in bacteria and are involved in time-of-day behavior or responses. However, their biological and regulatory role in non-photosynthetic bacteria is poorly understood, and even less is known about how they regulate diverse cellular processes. Here, we show that a bacteriophytochrome (XooBphP) from the plant pathogen Xanthomonas oryzae pv. oryzae perceives light signals and transduces a signal through its EAL-mediated phosphodiesterase activity, modulating the intracellular level of the ubiquitous bacterial second messenger c-di-GMP. We discover that light-mediated fine-tuning of intracellular c-di-GMP levels by XooBphP regulates production of virulence functions, iron metabolism, and transition from a sessile to a free-swimming motile lifestyle, contributing to its colonization of the host and virulence. XooBphP thus plays a crucial role in integrating photo-sensing with intracellular signaling to control the pathogenic lifestyle and social behavior.
细菌菌视紫红质是细菌中最丰富和普遍存在的光感受器，参与了生物钟行为或反应。然而，它们在非光合细菌中的生物学和调节作用还知之甚少，甚至不知道它们如何调节多样化的细胞过程。在这里，我们表明，来自水稻白叶枯病菌的一种细菌菌视紫红质（XooBphP）感知光信号，并通过其 EAL 介导的磷酸二酯酶活性传递信号，调节普遍存在的细菌第二信使 c-di-GMP 的细胞内水平。我们发现，XooBphP 通过光介导的细胞内 c-di-GMP 水平的精细调节，调节毒力功能、铁代谢和从静止到自由游动的游动生活方式的转换，从而有助于其对宿主的定植和毒力。因此，XooBphP 在将光感应与细胞内信号整合以控制致病生活方式和社会行为方面发挥着关键作用。
