Lipolytic gene DAGLA is targeted by miR-223 in chicken hepatocytes.

miR-223 is an important miRNA. It plays important roles in lipid metabolism by targeting related genes in mammals. It may be related to fatty liver in laying hens and its functions and target genes need further study. Through bioinformatics, we found that 349 genes were predicted as target genes of miR-223. Lipid-related gene DAGLA was among the predicted target genes. Dual-luciferase reporter assays showed that DAGLA was the target gene of miR-223 and the site mutation assays validated the target site of miR-223 in DAGLA. Overexpression of miR-223 in chicken hepatocytes LMH decreased the mRNA and protein expression of DAGLA, while knockdown of miR-223 increased expression of DAGLA in LMH cells, further indicating that miR-223 targets DAGLA and downregulates its expression. Since the target site of miR-223 in chicken DAGLA is not conserved, these findings suggest that miR-223 plays a specific role in chicken liver by regulating expression of target gene DAGLA.