Corporación Colombiana de Investigación Agropecuaria-AGROSAVIA, Research Center Motilonia, Km 5 vía a Becerril, Agustín Codazzi 202050, Colombia.
Faculty of Health, Microbiology Program, Universidad Popular del Cesar, Research Group in Parasitology-Millennium Agroecology, Valledupar 200002, Colombia.
Molecules. 2020 Sep 28;25(19):4447. doi: 10.3390/molecules25194447.
One of the principal etiological agents associated with losses in horticultural crops is the fungus sp. This study aimed to evaluate the in vitro effectiveness of the essential oil (EO) from in chitosan supports for the control of isolated from sweet pepper plants.
The extraction and phytochemical analysis of the EO of were performed along with its encapsulation in chitosan-agar in order to compare it with other techniques and determine its effect on .
The EO from the citral chemotype (58%) encapsulated in the chitosan-agar, with an 83% encapsulation efficiency in mass percentage, resulted in the total inhibition of mycelial growth at a minimum inhibitory concentration of 1370 ppm. This concentration was effective in controlling the disease under greenhouse conditions. The effectivity of the capsules containing EO was superior to that of other controls using EO evaluated in vitro. The capsules demonstrated an effective period of 51 days, with an additional 30 days of effectiveness after a reinfection cycle, thus providing similar results to the control with sp.
Chitosan capsules present a promising strategy in the use of EO on , and they are highly effective and stable under in vitro and field conditions.
与园艺作物损失相关的主要病因之一是真菌 sp. 本研究旨在评估来自 的精油(EO)在壳聚糖载体中的体外有效性,以控制从甜椒植物中分离出的 sp.
对 的 EO 进行提取和植物化学分析,并将其包封在壳聚糖-琼脂中,以便与其他技术进行比较,并确定其对 sp. 的影响。
用壳聚糖-琼脂包封的柠檬醛化学型(58%)的 EO,其质量百分比的包封效率为 83%,在最低抑制浓度为 1370 ppm 时导致菌丝生长完全抑制。该浓度在温室条件下有效控制了疾病。含有 EO 的胶囊的效果优于在体外评估的其他 EO 对照物。胶囊的有效期限为 51 天,在重新感染循环后又有 30 天的有效期限,因此与使用 sp. 的对照物的结果相似。
壳聚糖胶囊在使用 EO 方面提供了一种有前途的策略,在体外和田间条件下,它们具有高度的有效性和稳定性。