Lai Francesco, Wissing Sylvia A, Müller Rainer H, Fadda Anna M
Dipartimento Farmaco Chimico Tecnologico, Universitá degli Studi di Cagliari, Via Ospedale 72, 09124, Cagliari, Italy.
Department of Pharmaceutical Technology, Biotechnology and Quality Management, The Free University of Berlin, Kelchstr, 31, D-12169, Berlin, Germany.
AAPS PharmSciTech. 2006 Mar;7(1):E10. doi: 10.1208/pt070102. Epub 2017 Mar 8.
The aim of this study was to formulate a new delivery system for ecological pesticides by the incorporation of Artemisia arborescens L essential oil into solid lipid nanoparticles (SLN). Two different SLN formulations were prepared following the high-pressure homogenization technique using Compritol 888 ATO as lipid and Poloxamer 188 or Miranol Ultra C32 as surfactants. The SLN formulation particle size was determined using Photon correlation spectroscopy (PCS) and laser diffraction analysis (LD). The change of particle charge was studied by zeta potential (ZP) measurements, while the melting and recrystallization behavior was studied using differential scanning calorimetry (DSC). In vitro release studies of the essential oil were performed at 35°C. Data showed a high physical stability for both formulations at various storage temperatures during 2 months of investigation. In particular, average diameter of Artemisia arborescens L essential oil-loaded SLN did not vary during storage and increased slightly after spraying the SLN dispersions. In vitro release experiments showed that SLN were able to reduce the rapid evaporation of essential oil if compared with the reference emulsions. Therefore, obtained results showed that the studied SLN formulations are suitable carriers in agriculture.
本研究的目的是通过将树蒿艾精油掺入固体脂质纳米粒（SLN）中来制备一种新型生态农药递送系统。采用高压均质技术，以Compritol 888 ATO为脂质，泊洛沙姆188或Miranol Ultra C32为表面活性剂，制备了两种不同的SLN制剂。使用光子相关光谱法（PCS）和激光衍射分析（LD）测定SLN制剂的粒径。通过zeta电位（ZP）测量研究颗粒电荷的变化，同时使用差示扫描量热法（DSC）研究熔化和重结晶行为。在35°C下进行精油的体外释放研究。数据表明，在2个月的研究期间，两种制剂在不同储存温度下均具有较高的物理稳定性。特别是，负载树蒿艾精油的SLN的平均直径在储存期间没有变化，在喷洒SLN分散体后略有增加。体外释放实验表明，与参考乳液相比，SLN能够减少精油的快速蒸发。因此，所得结果表明，所研究的SLN制剂是农业中合适的载体。
