Mauduit G, Vincent C, Gielen V, Faure M, Demidem A, Thivolet J
Tissue Antigens. 1987 Feb;29(2):65-70. doi: 10.1111/j.1399-0039.1987.tb01553.x.
In vitro grown class II-MHC antigen free epidermal sheets were used as epidermal allografts (EAG) across a major histocompatibility barrier in 20 non-immunosuppressed recipients suffering from leg ulcers. Class I antigens were expressed on cell membranes of basal cell layer only on the epidermal sheets. After grafting, patchy areas of membrane fluorescence were observed among cells from the suprabasal layers on the epidermis from skin biopsies taken between days 5 and 28. All cells of the basal and the suprabasal layers expressed class I antigens on biopsies taken after day 28, as on normal human epidermis. This work demonstrates that class I antigens are expressed by epidermal cells in cultures used for grafting. The absence of rejection cannot be explained by the absence of class I-MHC antigens in EAG.
在体外培养的无II类主要组织相容性复合体抗原的表皮片被用作表皮异体移植物（EAG），移植到20名患有腿部溃疡且未接受免疫抑制的受体体内，跨越主要组织相容性屏障。I类抗原仅在表皮片的基底细胞层细胞膜上表达。移植后，在第5天至28天期间取自皮肤活检的表皮上，基底上层细胞之间观察到膜荧光的斑片状区域。在第28天后取自活检的样本中，基底和基底上层的所有细胞均如正常人表皮一样表达I类抗原。这项工作表明，用于移植的培养物中的表皮细胞表达I类抗原。EAG中I类主要组织相容性复合体抗原的缺失无法解释其未发生排斥反应的原因。
