未固定的材料包埋于甲基丙烯酸酯树脂（Technovit 7100）中用于免疫荧光染色。
Unfixed material embedded in a methacrylate resin (Technovit 7100) for immunofluorescent staining.
作者信息
Popp W, Zwick H
出版信息
Stain Technol. 1987 Mar;62(2):73-5. doi: 10.3109/10520298709107971.
PMID:3299880
Abstract
Wheat grains were embedded with or without fixation in Technovit 7100 or in paraffin. This enabled us to produce 4 microns sections for immunofluorescent staining to see whether serum of patients with baker's asthma contained IgG antibodies against wheat grains. Embedding without fixation in Technovit 7100 appeared to be suitable for immunofluorescent staining and gave superior results to protocols requiring fixation.
摘要
小麦粒在有或没有固定的情况下包埋于Technovit 7100或石蜡中。这使我们能够制作4微米厚的切片用于免疫荧光染色，以观察面包师哮喘患者的血清中是否含有针对小麦粒的IgG抗体。在Technovit 7100中不经过固定进行包埋似乎适用于免疫荧光染色，并且比需要固定的方案产生更好的结果。
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