Albrecht-Buehler G
Cell. 1977 Jun;11(2):395-404. doi: 10.1016/0092-8674(77)90057-5.
This paper describes a technique of visualizing tracks of cultured cells moving on a glass substrate covered with gold particles. It leads to the following observations: -If the tracks of many cells are examined, cell line characteristic track patterns become apparent. -In several cases, second or third generation descendents of 3T3 cells were observed to repeat track patterns of their ancestor cell. -If a 3T3 cell collides with another 3T3 cell or a nonmigrating BSC-1 cell, it forms an outgoing track from the impact area as if the cell was elastically reflected at the target.
本文描述了一种可视化培养细胞在覆盖有金颗粒的玻璃基板上移动轨迹的技术。由此得出以下观察结果:- 如果检查许多细胞的轨迹,细胞系特征性的轨迹模式就会变得明显。- 在几种情况下,观察到3T3细胞的第二代或第三代后代重复其祖细胞的轨迹模式。- 如果一个3T3细胞与另一个3T3细胞或一个不迁移的BSC-1细胞碰撞,它会从碰撞区域形成一条向外的轨迹,就好像细胞在靶点处发生了弹性反射一样。