Nichols M E, Rosenfield R E, Rubinstein P
Vox Sang. 1987;52(3):231-5. doi: 10.1111/j.1423-0410.1987.tb03034.x.
Mouse hybridoma clones have produced monoclonal antibodies directed against the K:14 and K:2 high-incidence antigens of the Kell blood group system. Two examples of anti-K14 were isolated, each arising from a separate fusion procedure. All three monoclonal antibodies are of the immunoglobulin class IgG1. Serological activity is consistent with that seen with human antibodies to high-incidence Kell system antigens, and their epitopes are destroyed, as usual, by 2-aminoethylisothiuronium bromide treatment. Specificity was further confirmed by adsorption and elution studies. Tests against nonhuman primate red cells demonstrated the expression of K:14 only by the great apes, whereas K:2 was present on all red cells tested. These findings emphasize the usefulness of monoclonal antibodies to elucidate the evolutionary patterns of blood group variants.
小鼠杂交瘤克隆产生了针对凯尔血型系统中K:14和K:2高频率抗原的单克隆抗体。分离出了两株抗K14的抗体，每株均来自独立的融合过程。所有三株单克隆抗体均为免疫球蛋白IgG1类。血清学活性与人类针对凯尔系统高频率抗原的抗体所表现出的活性一致，并且它们的表位像往常一样被2-氨基乙基异硫脲溴化物处理所破坏。通过吸附和洗脱研究进一步证实了其特异性。对非人类灵长类动物红细胞的检测表明，只有大猩猩表达K:14，而K:2存在于所有检测的红细胞上。这些发现强调了单克隆抗体在阐明血型变异进化模式方面的有用性。
