Parsons S F, Gardner B, Anstee D J
International Blood Group Reference Laboratory, South Western Regional Transfusion Centre, Bristol, U.K.
Transfus Med. 1993 Jun;3(2):137-42. doi: 10.1111/j.1365-3148.1993.tb00051.x.
Monoclonal antibodies BRIC 18, BRIC 68, BRIC 107 and BRIC 203 recognize high-frequency epitopes absent from erythrocytes expressing the Ko phenotype. BRIC 107 has anti-k (K2)-like specificity. BRIC 203 has a unique specificity denoted anti-Kpbc. All four monoclonal antibodies identify an M(r) 95,600 erythrocyte membrane protein by immunoprecipitation from radio-iodinated erythrocytes. In quantitative binding studies using IgG it is estimated that there are from 2000 (BRIC 18) to 4000 (BRIC 68) copies of the Kell glycoprotein per erythrocyte. Using Fab fragments the estimates are in the range 4000 (BRIC 18) to 18,000 (BRIC 68) copies. In competitive binding assays the four epitopes defined by the BRIC monoclonal antibodies fall into two non-overlapping groups. The first group comprises BRIC 18, BRIC 68, BRIC 203 and an antibody (6-22) with anti-K14 specificity. The second group contains BRIC 107 and two further anti-k-like monoclonal antibodies (BS45 and OSK5). The results suggest that the polymorphisms encoded at the K/k and Kpa/Kpb/Kpc loci may be located in two spatially distinct regions of the Kell glycoprotein(s).
单克隆抗体BRIC 18、BRIC 68、BRIC 107和BRIC 203识别表达Ko血型表型的红细胞所缺乏的高频表位。BRIC 107具有抗-k（K2）样特异性。BRIC 203具有一种独特的特异性，称为抗-Kpbc。通过对放射性碘化红细胞进行免疫沉淀，所有这四种单克隆抗体均识别出一种分子量为95,600的红细胞膜蛋白。在使用IgG的定量结合研究中，估计每个红细胞中有2000个（BRIC 18）至4000个（BRIC 68）拷贝的凯尔糖蛋白。使用Fab片段时，估计范围为4000个（BRIC 18）至18,000个（BRIC 68）拷贝。在竞争性结合试验中，由BRIC单克隆抗体定义的四个表位分为两个不重叠的组。第一组包括BRIC 18、BRIC 68、BRIC 203和一种具有抗-K14特异性的抗体（6-22）。第二组包含BRIC 107和另外两种抗-k样单克隆抗体（BS45和OSK5）。结果表明，在K/k和Kpa/Kpb/Kpc基因座编码的多态性可能位于凯尔糖蛋白的两个空间不同区域。
