School of Agriculture and Veterinary Medicine, University of Turin, Grugliasco, Turin, Italy.
Laboratorio La Vallonea, Passirana di Rho, Milano, Italy.
J Vet Diagn Invest. 2020 Nov;32(6):964-967. doi: 10.1177/1040638720963132. Epub 2020 Oct 1.
A 7-y-old mixed-breed male dog was presented with a history of generalized lymphadenopathy. Fine-needle aspirates of the enlarged peripheral lymph nodes were suggestive of lymphoma. Histologic examination of a retromandibular lymph node was suggestive of high-grade, medium large-cell lymphoma. Immunohistochemistry revealed concurrent expression of CD3 and CD20. The co-localization of the 2 antigens was confirmed by immunofluorescence. PCR for antigen receptor gene rearrangements (PARR) detected clonal rearrangements for both T-cell receptor gamma and B-cell receptor. The final diagnosis was CD3-CD20-positive anaplastic lymphoma with cross-lineage rearrangement.
一只 7 岁的混种雄性犬出现全身淋巴结肿大。外周淋巴结肿大的细针抽吸物提示为淋巴瘤。颌后淋巴结的组织学检查提示为高级别、中大型细胞淋巴瘤。免疫组织化学显示同时表达 CD3 和 CD20。免疫荧光证实了 2 种抗原的共定位。抗原受体基因重排（PARR）的 PCR 检测到 T 细胞受体γ和 B 细胞受体的克隆重排。最终诊断为 CD3-CD20 阳性间变性淋巴瘤，伴有跨谱系重排。
