School of Biosciences, University of Kent, Canterbury, CT2 7NJ, UK.
Chem Commun (Camb). 2020 Oct 7;56(78):11665-11668. doi: 10.1039/d0cc03612a. Epub 2020 Sep 2.
The activity of membrane proteins and compounds that interact with the membrane is modulated by the surrounding lipid composition. However, there are no simple methods that determine the composition of these annular phospholipids in eukaryotic systems. Herein, we describe a simple methodology that enables the identification and quantification of the lipid composition around membrane-associated compounds using SMA-nanodiscs and routine H-P NMR.
膜蛋白的活性和与膜相互作用的化合物的活性受周围脂质组成的调节。然而，在真核系统中，还没有确定这些环形磷脂的组成的简单方法。在此，我们描述了一种简单的方法，该方法使用 SMA-纳米盘和常规 H-P NMR 可以鉴定和定量与膜相关的化合物周围的脂质组成。
