包含 SMALPs 的大磁盘，用于膜蛋白的定向样品固态 NMR 光谱学。
Macrodiscs Comprising SMALPs for Oriented Sample Solid-State NMR Spectroscopy of Membrane Proteins.
机构信息
Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California.
Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California.
出版信息
Biophys J. 2018 Jul 3;115(1):22-25. doi: 10.1016/j.bpj.2018.05.024. Epub 2018 Jun 15.
Abstract
Macrodiscs, which are magnetically alignable lipid bilayer discs with diameters of >30 nm, were obtained by solubilizing protein-containing liposomes with styrene-maleic acid copolymers. Macrodiscs provide a detergent-free phospholipid bilayer environment for biophysical and functional studies of membrane proteins under physiological conditions. The narrow resonance linewidths observed from membrane proteins in styrene-maleic acid macrodiscs advance structure determination by oriented sample solid-state NMR spectroscopy.
摘要
大磁盘是指直径大于 30nm 的可磁化双层类脂盘，它是通过用苯乙烯-马来酸共聚物溶解含有蛋白质的脂质体而获得的。大磁盘为膜蛋白的生物物理和功能研究提供了一种无去污剂的磷脂双层环境，以模拟生理条件。在苯乙烯-马来酸大磁盘中，膜蛋白的共振线宽较窄，这有利于通过定向样品固态 NMR 光谱学来确定其结构。
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2
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Biochim Biophys Acta Biomembr. 2018 Mar;1860(3):777-783. doi: 10.1016/j.bbamem.2017.12.012. Epub 2017 Dec 19.
3
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4
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Methods Mol Biol. 2018;1700:83-96. doi: 10.1007/978-1-4939-7454-2_6.
6
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Biomacromolecules. 2017 Nov 13;18(11):3706-3713. doi: 10.1021/acs.biomac.7b01136. Epub 2017 Oct 9.
7
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8
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9
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10
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