Background Adiantum lunulatum Burm. F. leaf (AL) and its related species have been used traditionally for the treatment of various diseases. Objective The present study evaluated the hepatoprotective, and antioxidant activities of ethanolic extract of AL. Methodology and Result The hepatoprotective effect of AL was evaluated against ethanol-induced hepatotoxicity in rats. Administration of ethanol (2 g/kg) showed a significant biochemical and histological deterioration in the liver of experimental animals. Pretreatment with ethanolic extract of AL (250 and 500 mg/kg b.wt. p.o) significantly reduced the elevated levels of serum enzymes like serum glutamic-oxaloacetic transaminase (AST), serum glutamic-pyruvic transaminase (ALT), alkaline phosphatase (ALP), total protein, total bilirubin and reversed the hepatic damage in the liver which evidenced the hepatoprotective activity. The superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) level notably increased due to doses of AL. Conclusion The results of the present study demonstrate that the ethanolic extract of AL possesses hepatoprotective and antioxidant activities. Graphical Abstract.