Laamech Jawhar, El-Hilaly Jaouad, Fetoui Hamadi, Chtourou Yassine, Gouitaa Hanane, Tahraoui Adel, Lyoussi Badiaa
J Complement Integr Med. 2017 Mar 1;14(1). doi: 10.1515/jcim-2015-0079.
Background Berberis vulgaris L. (BV), commonly known as "Aghriss" in Moroccan pharmacopoeia, is used to cure liver disorders and other diseases. The present study aimed to investigate the protective effect of BV aqueous extract against lead-induced toxicity in mice liver. Methods Sixty IOPS mice were divided into six groups and were treated as follows: group 1 (normal control) received double distilled water; group 2 (toxic control) received lead acetate (5 mg/kg body weight/day) in double distilled water for 40 days; groups 3-6 received BV aqueous extract at doses of 25, 50, 100 and 150 mg/kg body weight , respectively, once daily for 30 days from 11 day after beginning of lead acetate exposure to the end of the experiment. Results Toxic control group showed a significant alteration of serum alanine-aminotransferase (ALT), aspartate-aminotransferase (AST), total cholesterol (TC), total bilirubin (TB), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) and reduced glutathione (GSH). Histological assessment of lead-intoxicated mice liver revealed alterations in hepatocytes and focal necrosis. BV treatment significantly prevented lead accumulation, increased ALT, AST, TC, and TB, inhibited lipid peroxidation and protein carbonyls(PCO) formation. Additionally, BV extract normalized the antioxidant enzymes (CAT, SOD and GPx), GSH and architecture of liver tissues. Conclusions BV aqueous extract exerts significant hepatoprotective effects against lead-induced oxidative stress and liver dysfunction. The BV effect may be mediated through the enhancement of antioxidant status, lead-chelating abilities and free radicals quenching.
背景 小檗（Berberis vulgaris L.，BV）在摩洛哥药典中通常被称为“Aghriss”，用于治疗肝脏疾病和其他疾病。本研究旨在探讨BV水提取物对铅诱导的小鼠肝脏毒性的保护作用。方法 将60只IOPS小鼠分为6组，进行如下处理：第1组（正常对照组）给予双蒸水；第2组（毒性对照组）给予醋酸铅（5毫克/千克体重/天），溶于双蒸水中，持续40天；第3 - 6组分别给予剂量为25、50、100和150毫克/千克体重的BV水提取物，从醋酸铅暴露开始后的第11天起，每天一次，持续30天直至实验结束。结果 毒性对照组血清丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、总胆固醇（TC）、总胆红素（TB）、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）和还原型谷胱甘肽（GSH）有显著变化。对铅中毒小鼠肝脏的组织学评估显示肝细胞有改变和局灶性坏死。BV处理显著阻止了铅的蓄积，增加了ALT、AST、TC和TB，抑制了脂质过氧化和蛋白质羰基（PCO）的形成。此外，BV提取物使抗氧化酶（CAT、SOD和GPx）、GSH以及肝组织结构恢复正常。结论 BV水提取物对铅诱导的氧化应激和肝功能障碍具有显著的肝脏保护作用。BV的作用可能是通过增强抗氧化状态、铅螯合能力和清除自由基来介导的。
