Department of Pharmacology, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, P.M.B. 12003 Lagos, Nigeria.
Department of Pharmacology, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, P.M.B. 12003 Lagos, Nigeria.
Chin J Nat Med. 2014 Apr;12(4):273-83. doi: 10.1016/S1875-5364(14)60054-6.
Hepatotoxicity is a significantly increasing health problem worldwide, and the extent of the problem has stimulated interest in the search for hepatotherapeutic agents from plants. This study investigated the hepatoprotective and in vivo antioxidant activities of the hydroethanolic extract of Mucuna pruriens leaves in antitubercular and alcohol-induced hepatotoxicity assays in rats.
In each of the models used, seven groups were allotted. The different groups received normal saline (10 mL·kg(-1), p.o.); hepatotoxicant (isoniazid-rifampicin, INH-RIF, 100 mg·kg(-1), i.p. or 20% ethanol 5 g·kg(-1), p.o.) and normal saline (10 mL·kg(-1), p.o.); hepatotoxicant and extract at doses of 100, 200, and 400 mg·kg(-1) p.o.; hepatotoxicant and silymarin 50 mg·kg(-1) p.o.; and extract at 400 mg·kg(-1) p.o. On the 21(st) day of treatment, blood was collected for assessment of serum biochemical parameters and harvested liver samples were assessed for antioxidants.
The hepatotoxicants significantly (P < 0.05-0.001) increased the levels of alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), bilirubin, and malondialdehyde (MDA); and reduced the levels of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and reduced glutathione GSH compared to control. M. pruriens significantly reversed (P < 0.05-0.001) the elevation in the level of ALT, AST, ALP, and bilirubin caused by the hepatotoxicants. The extract (200 and 400 mg·kg(-1)) significantly reversed (P < 0.05) the diminution in the level of in vivo antioxidants and increased the level of MDA produced by INH-RIF. M. pruriens (100-400 mg·kg(-1)) elicited significant reduction (P < 0.001) in the level of MDA compared to the alcohol group. Silymarin also reversed the deleterious effects of the hepatotoxicants.
The hydroethanolic extract of Mucuna pruriens leaves possesses hepatoprotective activity with enhancement of in vivo antioxidants as a possible mechanism of action.
肝毒性是一个在全球范围内日益严重的健康问题，其严重程度激发了人们对从植物中寻找肝治疗剂的兴趣。本研究旨在探讨在抗结核和酒精诱导的肝毒性模型中，黎豆叶的水乙醇提取物的肝保护和体内抗氧化活性。
在使用的每种模型中，都分配了七组。不同的组接受以下处理：生理盐水（10 mL·kg(-1)，p.o.）；肝毒性药物（异烟肼-利福平，INH-RIF，100 mg·kg(-1)，i.p.或 20%乙醇 5 g·kg(-1)，p.o.）和生理盐水（10 mL·kg(-1)，p.o.）；提取物剂量为 100、200 和 400 mg·kg(-1)，p.o.；肝毒性药物和水飞蓟素 50 mg·kg(-1)，p.o.；以及提取物 400 mg·kg(-1)，p.o.。在治疗的第 21 天，采集血液以评估血清生化参数，并采集肝组织样本以评估抗氧化剂。
肝毒性药物显著（P < 0.05-0.001）增加了丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、碱性磷酸酶（ALP）、胆红素和丙二醛（MDA）的水平；并降低了过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）和还原型谷胱甘肽（GSH）的水平，与对照组相比。黎豆叶显著逆转（P < 0.05-0.001）了肝毒性药物引起的 ALT、AST、ALP 和胆红素水平的升高。提取物（200 和 400 mg·kg(-1)）显著逆转（P < 0.05）了 INH-RIF 引起的体内抗氧化剂水平降低，并增加了 MDA 的水平。与酒精组相比，黎豆叶（100-400 mg·kg(-1)）显著降低（P < 0.001）了 MDA 的水平。水飞蓟素也逆转了肝毒性药物的有害作用。
黎豆叶的水乙醇提取物具有肝保护活性，并增强体内抗氧化剂，这可能是其作用机制。
