Martin-Luther-University Halle-Wittenberg, Institute of Agricultural and Nutritional Sciences, Weinbergweg 22, 06120 Halle/Saale, Germany.
Heinrich Heine University, Medical Faculty, Institute of Toxicology, Moorenstrasse 5, 40225 Düsseldorf, Germany.
Toxicol Lett. 2020 Nov 1;334:102-109. doi: 10.1016/j.toxlet.2020.09.016. Epub 2020 Sep 28.
Beauvericin is an ubiquitous mycotoxin with relevant occurrence in food and feed. It causes a high toxicity in several cell lines, but its general mechanism of action is not fully understood and only limited in vivo studies have been performed. We used Caenorhabditis elegans as a model organism to investigate effects of beauvericin. The mycotoxin displays a moderate acute toxicity at 100 μM; at this concentration also reproductive toxicity occurred (reduction of total progeny to 32.1 %), developmental toxicity was detectable at 250 μM. However, even lower concentrations were capable to reduce stress resistance and life span of the nematode: A significant reduction was detected at 10 μM beauvericin (decrease in mean survival time of 4.3 % and reduction in life span of 12.9 %). An increase in lipofuscin fluorescence was demonstrated starting at 10 μM suggesting oxidative stress as a mechanism of beauvericin toxicity. Beauvericin (100 μM) increases the number of apoptotic germ cells comparable to the positive control UV-C (400 J/m). Conclusion: Low concentrations of beauvericin are capable to cause adverse effects in C. elegans, which may be relevant for hazard identification of this compound.
beauvericin 是一种普遍存在的真菌毒素，在食品和饲料中有相关的发生。它在几种细胞系中引起高毒性，但它的一般作用机制尚未完全了解，只有有限的体内研究已经进行。我们使用秀丽隐杆线虫作为模型生物来研究 beauvericin 的作用。该真菌毒素在 100 μM 时表现出中等急性毒性；在这个浓度下也发生生殖毒性（总后代减少到 32.1%），在 250 μM 时可检测到发育毒性。然而，即使更低的浓度也能够降低线虫的应激抗性和寿命：在 10 μM beauvericin 时检测到显著降低（平均存活时间减少 4.3%，寿命减少 12.9%）。从 10 μM 开始，证明了脂褐素荧光的增加，表明氧化应激是 beauvericin 毒性的一种机制。beauvericin（100 μM）增加了与阳性对照 UV-C（400 J/m）相当数量的凋亡生殖细胞。结论：低浓度的 beauvericin 能够在 C. elegans 中引起不良反应，这可能与该化合物的危害识别有关。
