Drost R H, Plomp T A, Teunissen A J, Maes A H, Maes R A
Clin Chim Acta. 1977 Sep 15;79(3):557-67. doi: 10.1016/0009-8981(77)90176-0.
Our experience with the determination of digoxin in plasma or serum using a homogeneous enzyme immunoassay technique (EMIT) is reported. The day-to-day precision of the EMIT digoxin assay was investigated with different series of calibrators. Coefficients of variation varied from 10 to 25 percent in the range of 0.65--7.0 ng digoxin/ml. The accuracy was established by determining the mean recovery (96 percen) of spiked serum samples (0.0--6.0 ng digoxin/ml). The cross reactivity of structure related compounds: digitoxin, spironolactone (Aldactone) and prednisone were investigated. Amniotic fluid, umbilical cord serum and serum of pregnant patients were examined for false positive reaction. Serum samples of 111 patients from two hospitals, who were treated with digoxin, were analysed by EMIT and 3H-radioimmunoassay (RIA); 38 of these samples were also determined by 125I-RIA. A good correlation was found between EMIT assay and these techniques (r =0.90 and 0.91, respectively).
本文报道了我们使用均相酶免疫分析技术（EMIT）测定血浆或血清中地高辛的经验。使用不同系列的校准物研究了EMIT地高辛测定法的日常精密度。在0.65--7.0 ng地高辛/毫升范围内，变异系数在10%至25%之间。通过测定加标血清样品（0.0--6.0 ng地高辛/毫升）的平均回收率（96%）来确定准确性。研究了结构相关化合物洋地黄毒苷、螺内酯（安体舒通）和泼尼松的交叉反应性。检测羊水、脐带血清和孕妇血清是否存在假阳性反应。用EMIT和3H放射免疫分析法（RIA）分析了两家医院111例接受地高辛治疗患者的血清样本；其中38份样本也用125I-RIA进行了测定。发现EMIT测定法与这些技术之间具有良好的相关性（分别为r = 0.90和0.91）。
