Institute of Biomedical Sciences, Academia Sinica, Taipei 115, Taiwan.
Institute of Biomedical Sciences, Academia Sinica, Taipei 115, Taiwan.
Colloids Surf B Biointerfaces. 2020 Dec;196:111364. doi: 10.1016/j.colsurfb.2020.111364. Epub 2020 Sep 22.
Medical devices are widely used in modern medicine, but their utilities are often limited by the biofilm formation of bacteria that are tolerant to most antibiotics. In this report, antimicrobial peptides (AMPs) were coated onto biomaterials by the aid of surfactant through hydrophobic interactions. To increase the coating efficiency, stability of AMPs in body fluids and spectrum of antimicrobial activity, pairs of AMPs were coated simultaneously onto various substrates, such as silicone, polyurethane and titanium, which are commonly used components of biomedical devices. These coated AMPs exhibited very low cytotoxicity and hemolytic activities because they were gradually released into urine or serum. The AMP pairs, such as T9W + SAAP159 and T9W + RRIKA, coated onto the silicone discs were able to inhibit in vitro bacterial adherence in urine. Most importantly, AMP pairs coated onto the silicone tubing by surfactant SDBS could prevent bacterial adherence to mouse bladder and the silicone tubing implanted within it. These results provide a promising approach towards circumventing urinary catheter-associated infections caused by bacterial adherence.
医疗器械在现代医学中得到了广泛应用，但它们的效用常常受到细菌生物膜形成的限制，这些细菌对大多数抗生素具有耐受性。在本报告中，抗菌肽（AMPs）通过疏水相互作用在生物材料上涂覆表面活性剂。为了提高涂层效率、增加 AMP 在体液中的稳定性和扩大抗菌谱，我们将成对的 AMP 同时涂覆到各种基质上，如硅酮、聚氨酯和钛，这些都是生物医学设备中常用的组件。这些涂覆的 AMP 表现出非常低的细胞毒性和溶血活性，因为它们逐渐释放到尿液或血清中。涂覆在硅酮圆盘上的 AMP 对，如 T9W + SAAP159 和 T9W + RRIKA，能够抑制尿液中的体外细菌黏附。最重要的是，表面活性剂 SDBS 涂覆在硅酮管上的 AMP 对可以防止细菌黏附到小鼠膀胱和植入的硅酮管上。这些结果为避免由细菌黏附引起的导尿管相关感染提供了一种很有前途的方法。
