Wang Shih-Han, Tang Tony Wen-Hung, Wu Eden, Wang Dan-Wei, Liao You-Di
Institute of Biomedical Sciences, Academia Sinica, Taipei 115, Taiwan.
ACS Biomater Sci Eng. 2020 Aug 10;6(8):4561-4572. doi: 10.1021/acsbiomaterials.0c00716. Epub 2020 Jul 30.
Medical devices are widely used in modern medicine, but the high prevalence of biomaterial-associated infections still presents a major problem. Especially problematic is the formation of biofilms that are tolerant to most antibiotics. In this report, antimicrobial peptides (AMPs) were driven into an amphipathic structure by anionic surfactant. To increase the coating efficacy and spectrum of antimicrobial activity, the AMPs were coated simultaneously with antibiotic, Polymyxin B, by surfactant onto polystyrene, silicone, polyurethane, and titanium which are commonly used with biomedical devices. These coated antimicrobials stably adhered to the substrate and were gradually released into urine and serum. They exhibited high bactericidal activity, but low cytotoxicity and hemolytic activity. Most importantly, the antimicrobials coated onto silicone tubing inhibited the planktonic growth of in mouse urine and also markedly prevented bacterial adherence to the bladder and the silicone tubing implanted in the bladder. These results provide a promising approach to circumvent catheter-associated infections due to bacterial adherence.
医疗设备在现代医学中被广泛使用，但生物材料相关感染的高发生率仍然是一个主要问题。特别成问题的是对大多数抗生素具有耐受性的生物膜的形成。在本报告中，抗菌肽（AMPs）通过阴离子表面活性剂被驱动形成两亲结构。为了提高包被效率和抗菌活性谱，AMPs与抗生素多粘菌素B同时被表面活性剂包被在常用于生物医学设备的聚苯乙烯、硅酮、聚氨酯和钛上。这些包被的抗菌剂稳定地附着在基质上，并逐渐释放到尿液和血清中。它们表现出高杀菌活性，但细胞毒性和溶血活性低。最重要的是，包被在硅胶管上的抗菌剂抑制了小鼠尿液中细菌的浮游生长，还显著防止了细菌粘附到膀胱以及植入膀胱的硅胶管上。这些结果为规避因细菌粘附导致的导管相关感染提供了一种有前景的方法。
