Mészáros K, Bagby G J, Lang C H, Spitzer J J
Am J Physiol. 1987 Jul;253(1 Pt 1):E33-9. doi: 10.1152/ajpendo.1987.253.1.E33.
Glucose metabolism of respiratory and nonrespiratory muscles of different fiber composition was investigated in conscious rats. The accumulation of phosphorylated 2-deoxyglucose (2DGP) was increased in skeletal muscles by 56-102% and in diaphragm by 236% at 3 h after treatment with 100 micrograms/100 g endotoxin. The increase was still marked at 24 h, whereas it diminished at 48 h in the diaphragm, abdominal muscle, and white portion of the quadriceps. In the red portion of this muscle 2DGP accumulation was less than that in time-matched controls at 24 and 48 h. Whole gastrocnemius (mixed-fiber types) showed no changes after 24 h. The high 2DGP accumulation in brain remained stable. The retention of 2DGP in tissues, studied by sequential double labeling, did not change 3 h after endotoxin. The lumped constant was similar in the isolated epitrochlear muscles of endotoxemic and control rats. Whole-body glucose utilization (Rd) was increased by 68% 3 h after endotoxin, but it was normal at 24 and 48 h. The increase of glucose utilization by the entire skeletal muscle mass was responsible for approximately 25% of the increase in Rd; therefore it appears that other tissues also contributed significantly to the endotoxin-induced alterations in carbohydrate metabolism.
在清醒大鼠中研究了不同纤维组成的呼吸肌和非呼吸肌的葡萄糖代谢。用100微克/100克内毒素处理3小时后，骨骼肌中磷酸化2-脱氧葡萄糖（2DGP）的积累增加了56%-102%，膈肌中增加了236%。24小时时增加仍很明显，而在48小时时，膈肌、腹肌和股四头肌白色部分的增加有所减少。在该肌肉的红色部分，24小时和48小时时2DGP的积累低于时间匹配的对照组。24小时后，整个腓肠肌（混合纤维类型）没有变化。脑中高2DGP积累保持稳定。通过顺序双重标记研究的组织中2DGP的滞留在内毒素处理3小时后没有变化。内毒素血症大鼠和对照大鼠的离体肱三头肌的集总常数相似。内毒素处理3小时后，全身葡萄糖利用率（Rd）增加了68%，但在24小时和48小时时正常。整个骨骼肌质量的葡萄糖利用率增加约占Rd增加的25%；因此，似乎其他组织也对内毒素诱导的碳水化合物代谢改变有显著贡献。
