Nuutila P, Koivisto V A, Knuuti J, Ruotsalainen U, Teräs M, Haaparanta M, Bergman J, Solin O, Voipio-Pulkki L M, Wegelius U
Department of Medicine, University of Turku, Finland.
J Clin Invest. 1992 Jun;89(6):1767-74. doi: 10.1172/JCI115780.
Positron emission tomography permits noninvasive measurement of regional glucose uptake in vivo in humans. We employed this technique to determine the effect of FFA on glucose uptake in leg, arm, and heart muscles. Six normal men were studied twice under euglycemic hyperinsulinemic (serum insulin approximately 500 pmol/liter) conditions, once during elevation of serum FFA by infusions of heparin and Intralipid (serum FFA 2.0 +/- 0.4 mmol/liter), and once during infusion of saline (serum FFA 0.1 +/- 0.01 mmol/liter). Regional glucose uptake rates were measured using positron emission tomography-derived 18F-fluoro-2-deoxy-D-glucose kinetics and the three-compartment model described by Sokoloff (Sokoloff, L., M. Reivich, C. Kennedy, M. C. Des Rosiers, C. S. Patlak, K. D. Pettigrew, O. Sakurada, and M. Shinohara. 1977. J. Neurochem. 28: 897-916). Elevation of plasma FFA decreased whole body glucose uptake by 31 +/- 2% (1,960 +/- 130 vs. 2,860 +/- 250 mumol/min, P less than 0.01, FFA vs. saline study). This decrease was due to inhibition of glucose uptake in the heart by 30 +/- 8% (150 +/- 33 vs. 200 +/- 28 mumol/min, P less than 0.02), and in skeletal muscles; both when measured in femoral (1,594 +/- 261 vs. 2,272 +/- 328 mumol/min, 25 +/- 13%) and arm muscles (1,617 +/- 411 to 2,305 +/- 517 mumol/min, P less than 0.02, 31 +/- 6%). Whole body glucose uptake correlated with glucose uptake in femoral (r = 0.75, P less than 0.005), and arm muscles (r = 0.69, P less than 0.05) but not with glucose uptake in the heart (r = 0.04, NS). These data demonstrate that the glucose-FFA cycle operates in vivo in both heart and skeletal muscles in humans.
正电子发射断层扫描术可对人体体内局部葡萄糖摄取进行无创测量。我们运用该技术来确定游离脂肪酸（FFA）对腿部、手臂及心肌葡萄糖摄取的影响。对6名正常男性在正常血糖高胰岛素血症（血清胰岛素约500 pmol/升）条件下进行了两次研究，一次是在通过输注肝素和英脱利匹特使血清游离脂肪酸升高时（血清游离脂肪酸2.0±0.4 mmol/升），另一次是在输注生理盐水时（血清游离脂肪酸0.1±0.01 mmol/升）。使用正电子发射断层扫描衍生的18F - 氟 - 2 - 脱氧 - D - 葡萄糖动力学及索科洛夫描述的三室模型（索科洛夫，L.，M. 雷维奇，C. 肯尼迪，M. C. 德罗斯耶，C. S. 帕特拉克，K. D. 佩蒂格鲁，O. 樱田，和M. 筱原。1977年。《神经化学杂志》28: 897 - 916）测量局部葡萄糖摄取率。血浆游离脂肪酸升高使全身葡萄糖摄取降低了31±2%（1960±130对2860±250 μmol/分钟，P＜0.01，游离脂肪酸组对比生理盐水组研究）。这种降低是由于心脏葡萄糖摄取受抑制30±8%（150±33对200±28 μmol/分钟，P＜0.02），以及骨骼肌葡萄糖摄取受抑制；在股部肌肉测量时（1594±261对2272±328 μmol/分钟，25±13%）和手臂肌肉测量时（1617±411至2305±517 μmol/分钟，P＜0.02，31±6%）均如此。全身葡萄糖摄取与股部肌肉（r = 0.75，P＜0.005）和手臂肌肉（r = 0.69，P＜0.05）的葡萄糖摄取相关，但与心脏葡萄糖摄取无关（r = 0.04，无显著性差异）。这些数据表明，葡萄糖 - 游离脂肪酸循环在人体心脏和骨骼肌中均在体内发挥作用。
