应用下一代测序技术在脑膜脑炎患者中检测单核细胞增生李斯特菌：病例报告。
Detection of Listeria monocytogenes in a patient with meningoencephalitis using next-generation sequencing: a case report.
机构信息
The Second Xiangya Hospital, Central South University, No.139 Middle Renmin Road, Changsha, 410011, Hunan, China.
Hugobiotech Co., Ltd, No 1 Disheng East Road, Daxing District, Beijing, 100000, China.
出版信息
BMC Infect Dis. 2020 Oct 1;20(1):721. doi: 10.1186/s12879-020-05447-z.
BACKGROUND
Listeria monocytogenes (L. monocytogenes) is a facultative intracellular bacterial pathogen which can invade different mammalian cells and reach to the central nervous system (CNS), leading to meningoencephalitis and brain abscesses. In the diagnosis of L. monocytogenes meningoencephalitis (LMM), the traditional test often reports negative owing to the antibiotic treatment or a low number of bacteria in the cerebrospinal fluid. To date, timely diagnosis and accurate treatment remains a challenge for patients with listeria infections.
CASE PRESENTATION
We present the case of a 66-year-old woman whose clinical manifestations were suspected as tuberculous meningoencephalitis, but the case was finally properly diagnosed as LMM by next-generation sequencing (NGS). The patient was successfully treated using a combined antibacterial therapy, comprising ampicillin and trimethoprim-sulfamethoxazole.
CONCLUSION
To improve the sensitivity of LMM diagnosis, we used NGS for the detection of L. monocytogenes. Hence, the clinical utility of this approach can be very helpful since it provides quickly and trust results.
背景
单核细胞增生李斯特菌（Listeria monocytogenes，L. monocytogenes）是一种兼性细胞内细菌病原体，能够侵入不同的哺乳动物细胞并进入中枢神经系统（CNS），导致脑膜脑炎和脑脓肿。在单核细胞增生李斯特菌脑膜脑炎（LMM）的诊断中，由于抗生素治疗或脑脊液中细菌数量较少，传统检测往往报告为阴性。迄今为止，对于李斯特菌感染的患者，及时诊断和准确治疗仍然是一个挑战。
病例介绍
我们报告了一例 66 岁女性的病例，其临床表现疑似结核性脑膜脑炎，但最终通过下一代测序（NGS）被正确诊断为 LMM。该患者成功接受氨苄西林和复方磺胺甲噁唑联合抗菌治疗。
结论
为了提高 LMM 诊断的灵敏度，我们使用 NGS 检测 L. monocytogenes。因此，这种方法的临床实用性非常有帮助，因为它可以提供快速且可靠的结果。
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