Zhu Tong-Yin, Ai Jing, Nie Chun-Hui, Zhou Guan-Hui, Chen Xin-Hua, Zhang Yue-Lin, Zhou Tan-Yang, Chen Sheng-Qun, Wang Bao-Quan, Zheng Shu-Sen, Wu Li-Ming, Sun Jun-Hui
Division of Hepatobiliary and Pancreatic Surgery, Hepatobiliary and Pancreatic Interventional Treatment Center, The First Affiliated Hospital, Zhejiang University School of Medicine; Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health; Collaborative Innovation Center for Diagnosis Treatment of Infectious Diseases, Zhejiang University, Hangzhou, China.
Eye Centre, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
J Cancer Res Ther. 2020 Sep;16(5):1125-1128. doi: 10.4103/jcrt.JCRT_594_19.
The objective was to evaluate the feasibility and safety of computed tomography (CT)-guided percutaneous irreversible electroporation (IRE) in porcine kidneys.
Under CT guidance, two monopole probes were used to precisely puncture through the renal parenchyma into the renal hilum in nine anesthetized adult Bama miniature pigs. After which, IRE ablation was performed. Biochemical and pathological examinations were carried out 2 h, 2, 7, and 14 days after the procedure.
All procedures were performed successfully without any serious complications such as bleeding, infection, or death. All pigs survived until the end of the study. Pathological examinations showed that cells in the ablation area were dead within 2 days after the procedure, whereas the vascular endothelium showed only slight damage. After 2 days, endothelialization ensued and regrowth of smooth muscle cells was observed after 14 days. Hemogram tests indicated a transient increase but gradually returned to baseline levels 14 days after the procedure.
IRE was essentially safe, however further studies on tumor ablation using several different animal models are needed.
评估计算机断层扫描（CT）引导下经皮不可逆电穿孔（IRE）在猪肾中的可行性和安全性。
在CT引导下，使用两个单极探针精确穿刺9只麻醉的成年巴马小型猪的肾实质进入肾门。之后，进行IRE消融。在术后2小时、2天、7天和14天进行生化和病理检查。
所有手术均成功完成，无出血、感染或死亡等严重并发症。所有猪均存活至研究结束。病理检查显示，术后2天内消融区域的细胞死亡，而血管内皮仅显示轻微损伤。2天后发生内皮化，14天后观察到平滑肌细胞再生。血常规检查显示短暂升高，但术后14天逐渐恢复至基线水平。
IRE基本安全，但需要使用几种不同动物模型对肿瘤消融进行进一步研究。
