Faculty of Dentistry, National University of Singapore, 9 Lower Kent Ridge Road, Singapore, 119085, Singapore.
Exocrine Gland Biology and Regeneration Research Group, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.
Sci Rep. 2020 Oct 1;10(1):16189. doi: 10.1038/s41598-020-73292-w.
Oral mucositis (OM) is a common complication of cancer therapy, however OM management remains unsatisfactory. There is a growing interest in the therapeutic potential of probiotics in OM due to positive findings of its use in intestinal mucositis. This study aimed to determine the efficacy and safety of the probiotic combination Lactobacillus reuteri DSM 17938 and ATCC PTA 5289 strains in chemotherapy-induced OM. Mice were divided into 4 groups. PBS/water and PBS/LR groups comprised of mice injected with PBS intraperitoneally (i.p.), and were given water or the mixture of L. reuteri (LR) DSM 17938 and ATCC PTA 5289 in water respectively. The 5-FU/water and 5-FU/LR groups comprised of mice injected with 5-FU i.p., and were given water or L. reuteri DSM 17938 and ATCC PTA 5289 in water respectively. Histopathological analysis revealed that the oral epithelia of the 5-FU/water and 5-FU/LR groups were thinner compared to PBS/water and PBS/LR groups. However, epithelial damage was significantly reduced in the 5-FU/LR compared to 5-FU/water group. Additionally, the 5-FU/LR group showed reduced oxidative stress and inflammation in the oral mucosa. We further showed that L. reuteri reduced oxidative stress through the nuclear factor E2-related factor-2 (Nrf-2) signalling. There was no evidence of translocation of L. reuteri systemically. This study demonstrated for the first time that L. reuteri protected oral mucosa against damage induced by chemotherapy.
口腔黏膜炎（OM）是癌症治疗的常见并发症，但 OM 的管理仍不尽如人意。由于益生菌在肠道黏膜炎中的应用有积极的发现，因此人们对其在 OM 中的治疗潜力越来越感兴趣。本研究旨在确定益生菌组合乳杆菌 DSM 17938 和 ATCC PTA 5289 菌株在化疗诱导的 OM 中的疗效和安全性。将小鼠分为 4 组。PBS/水和 PBS/LR 组由腹腔内注射 PBS 的小鼠组成，分别给予水或 L. reuteri（LR）DSM 17938 和 ATCC PTA 5289 的混合物溶于水。5-FU/水和 5-FU/LR 组由腹腔内注射 5-FU 的小鼠组成，分别给予水或 L. reuteri DSM 17938 和 ATCC PTA 5289 的混合物溶于水。组织病理学分析显示，与 PBS/水和 PBS/LR 组相比，5-FU/水和 5-FU/LR 组的口腔上皮更薄。然而，与 5-FU/水组相比，5-FU/LR 组的上皮损伤明显减少。此外，5-FU/LR 组口腔黏膜的氧化应激和炎症减轻。我们进一步表明，L. reuteri 通过核因子 E2 相关因子 2（Nrf-2）信号通路减轻氧化应激。没有证据表明 L. reuteri 发生全身性移位。本研究首次证明 L. reuteri 可保护口腔黏膜免受化疗引起的损伤。
