Hu Weihua, Huang Peipei, Xiong Yang, Guo Wenjie, Wang Yuhong, Fan Qixue, Wang Qingyun, Mei Jie
College of Fisheries, Huazhong Agricultural University, Wuhan, China.
Fisheries Research Institute, Wuhan Academy of Agricultural Sciences, Wuhan, China.
Front Genet. 2020 Aug 28;11:961. doi: 10.3389/fgene.2020.00961. eCollection 2020.
Multiple repeat spawners make large contributions to long-term population stability and aquaculture breeding programs. A high percentage of female yellow catfish () died for spawning failure or incomplete spawning after artificial spawning by traditional synthetic hormones including human chorionic gonadotropin (hCG), luteinizing hormone releasing hormone (LHRH), and domperidone (DOM). The present study was designed to compare the efficacy of different combinations of exogenous hormones for inducing ovulation in yellow catfish using hCG, LHRH, DOM, and carp pituitary extraction (CPE). We found a optimal strategy for exogenous hormones administration, the mixture of LHRH/CPE for the first injection and LHRH/CPE/DOM for the second injection, could greatly improve the rates of spawning success, weight of ovulated eggs and survival rate after spawning. Interestingly, a population of female yellow catfish with defective reproductive duct could not spawn and showed high mortality after induced by a combination of hCG/LHRH/DOM, whereas a synergistic combination of hCG, LHRH, DOM, and CPE could efficiently induce spawning and reduce mortality in the defective yellow catfish, in which a significant decrease of Vitellin and E2 levels. Altogether, our findings provide an effective combination of exogenous hormones to improve spawning and post-spawning survival of female yellow catfish.
多次重复产卵者对长期种群稳定性和水产养殖育种计划有重大贡献。在使用包括人绒毛膜促性腺激素（hCG）、促黄体生成素释放激素（LHRH）和多潘立酮（DOM）在内的传统合成激素进行人工催产之后，高比例的雌性黄颡鱼因催产失败或催产不完全而死亡。本研究旨在比较使用hCG、LHRH、DOM和鲤鱼脑垂体提取物（CPE）的不同外源激素组合对黄颡鱼诱导排卵的效果。我们发现一种外源激素给药的最佳策略，即首次注射使用LHRH/CPE组合，第二次注射使用LHRH/CPE/DOM组合，可大大提高催产成功率、排卵卵重和产后存活率。有趣的是，一群生殖管道有缺陷的雌性黄颡鱼在使用hCG/LHRH/DOM组合诱导后无法产卵且死亡率很高，而hCG、LHRH、DOM和CPE的协同组合可有效诱导有缺陷的黄颡鱼产卵并降低死亡率，其中卵黄蛋白和雌二醇水平显著降低。总之，我们的研究结果提供了一种有效的外源激素组合，以提高雌性黄颡鱼的催产率和产后存活率。
