Liu Zengjin, Guo Shewei, Sun Hongwei, Bai Yahui, Song Zhenyu, Liu Xianzhi
Department of Neurosurgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Front Genet. 2020 Aug 28;11:1013. doi: 10.3389/fgene.2020.01013. eCollection 2020.
As a malignant tumor of the central nervous system, glioma exhibits high incidence and poor prognosis. Circular RNA HIPK3 (circHIPK3) is a circular RNA (circRNA) related to cancer progression. However, the role of circHIPK3 in gliomas remains unclear. The purpose of this study was to investigate the role of circHIPK3 in gliomas and its mechanism. The qRT-PCR method was used to determine the expression pattern of circHIPK3 in glioma cell lines. CCK-8 assay was used to detect cell proliferation. Cell migration and invasion were evaluated using the Transwell assay. Our results showed that circHIPK3 expression was significantly up-regulated in glioma tissues and cell lines. , the down-regulation of circHIPK3 significantly inhibited the proliferation, migration and invasion of glioma cells. Besides, we demonstrated that circHIPK3 acted as a sponge to absorb miR-124 and promoted CCND2 expression. In summary, our results indicated that circHIPK3 had carcinogenic effects by regulating the expression of CCND2 in glioma by sponging miR-124. These findings provided favorable evidence to reveal the role of circHIPK3 in the development of gliomas.
作为中枢神经系统的恶性肿瘤，胶质瘤具有高发病率和预后差的特点。环状RNA HIPK3（circHIPK3）是一种与癌症进展相关的环状RNA（circRNA）。然而，circHIPK3在胶质瘤中的作用仍不清楚。本研究的目的是探讨circHIPK3在胶质瘤中的作用及其机制。采用qRT-PCR方法检测circHIPK3在胶质瘤细胞系中的表达模式。使用CCK-8法检测细胞增殖。采用Transwell法评估细胞迁移和侵袭能力。我们的结果表明，circHIPK3在胶质瘤组织和细胞系中的表达显著上调。此外，circHIPK3的下调显著抑制了胶质瘤细胞的增殖、迁移和侵袭。此外，我们证明circHIPK3作为海绵吸附miR-124并促进CCND2表达。总之，我们的结果表明，circHIPK3通过海绵吸附miR-124调节胶质瘤中CCND2的表达而具有致癌作用。这些发现为揭示circHIPK3在胶质瘤发生发展中的作用提供了有力证据。
